Khansarinejad, Behzad, Soleimanjahi, Hooreih, Hamidieh, Amirali, Mirab Samiee, Siamak, Paryan, Mahdi, Sanahmadi, Yadollah, Faghihzadeh, Soghrat (2012). Comparison of Qualitative PCR and pp65 Antigenemia for the Diagnosis of CMV Infection in Hematopoietic Stem Cell Transplanted Patients. , 15(1), 13-22.
Behzad Khansarinejad; Hooreih Soleimanjahi; Amirali Hamidieh; Siamak Mirab Samiee; Mahdi Paryan; Yadollah Sanahmadi; Soghrat Faghihzadeh. "Comparison of Qualitative PCR and pp65 Antigenemia for the Diagnosis of CMV Infection in Hematopoietic Stem Cell Transplanted Patients". , 15, 1, 2012, 13-22.
Khansarinejad, Behzad, Soleimanjahi, Hooreih, Hamidieh, Amirali, Mirab Samiee, Siamak, Paryan, Mahdi, Sanahmadi, Yadollah, Faghihzadeh, Soghrat (2012). 'Comparison of Qualitative PCR and pp65 Antigenemia for the Diagnosis of CMV Infection in Hematopoietic Stem Cell Transplanted Patients', , 15(1), pp. 13-22.
Khansarinejad, Behzad, Soleimanjahi, Hooreih, Hamidieh, Amirali, Mirab Samiee, Siamak, Paryan, Mahdi, Sanahmadi, Yadollah, Faghihzadeh, Soghrat Comparison of Qualitative PCR and pp65 Antigenemia for the Diagnosis of CMV Infection in Hematopoietic Stem Cell Transplanted Patients. , 2012; 15(1): 13-22.

Comparison of Qualitative PCR and pp65 Antigenemia for the Diagnosis of CMV Infection in Hematopoietic Stem Cell Transplanted Patients

Pathobiology Reserach
Article 2, Volume 15, Issue 1, 2012, Pages 13-22    PDF (487.84 K)
Authors
Behzad Khansarinejad1; Hooreih Soleimanjahi* 1; Amirali Hamidieh2; Siamak Mirab Samiee3; Mahdi Paryan4; Yadollah Sanahmadi5; Soghrat Faghihzadeh6
1Department of Virology, Faculty of medical science, Tarbiat Modares University, Tehran, Iran
2Hematology Oncology and Stem Cell Transplantation Research Center, Tehran University of Medical Sciences, Tehran, Iran
3Food and Drug Laboratory Research Center, Ministry of Health and Medical Education, Tehran, Iran
4Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran
5MD, Day General Hospital Laboratory, Tehran, Iran
6Department of Biostatistics, Faculty of medical science, Tarbiat Modares University, Tehran, Iran
Abstract
Objectives: Human cytomegalovirus (CMV) is a major life-threatening pathogen for hematopoietic stem cell transplant recipients. Specific tests are used for the diagnosis and monitoring of CMV infection in transplant patients. This study evaluates the performance of pp65 antigenemia and qualitative PCR assays for monitoring CMV in such patients.
Methods: We analyzed 179 clinical samples from 41 patients by using a validated home-brewed qualitative PCR and a commercial antigenemia assay. The obtained results were evaluated using quantitative real-time PCR as the gold standard.
Results: CMV was observed in 26.8% of samples analyzed by the antigenemia assay and in 42.6% of the samples by qualitative PCR. Among 179 clinical samples, 50.8% were negative and 21.2% were positive by both assays. On the other hand, 26.3% were only positive by qualitative PCR whereas 1.7% were positive by the antigenemia assay. A comparison of the results with real-time PCR showed that qualitative PCR has a higher sensitivity than the antigenemia assay (98.7% vs. 45.7%). The specificity of both assays was equal (96.8%). Quantitative results of the antigenemia assay showed good correlation with real-time PCR (r=0.715; p<0.001).
Conclusion: Both the qualitative PCR and antigenemia assays have special deficiencies for efficient diagnosis of CMV infection. Therefore, effective management of CMV infection in transplant patients requires the use of other sensitive quantitative methods such as qPCR.
Keywords
PCR; Real-time PCR; pp64; Antigenemia; Cytomegalovirus; pp65
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