Solali, Saeid, Kaviani, Saeid, Movassaghpuor, Ali Akbar, Ali-parasti, Mohammad Reza, Soleimani, Masoud, Noruzinia, Mehrdad, Abroun, Saeid, Kaviani, Zeinab (2011). Quantitative evaluation of Imatib efflux and influx transporters expression in Chronic Myeloid Leukemia (CML) patients. , 14(1), 49-57.
Saeid Solali; Saeid Kaviani; Ali Akbar Movassaghpuor; Mohammad Reza Ali-parasti; Masoud Soleimani; Mehrdad Noruzinia; Saeid Abroun; Zeinab Kaviani. "Quantitative evaluation of Imatib efflux and influx transporters expression in Chronic Myeloid Leukemia (CML) patients". , 14, 1, 2011, 49-57.
Solali, Saeid, Kaviani, Saeid, Movassaghpuor, Ali Akbar, Ali-parasti, Mohammad Reza, Soleimani, Masoud, Noruzinia, Mehrdad, Abroun, Saeid, Kaviani, Zeinab (2011). 'Quantitative evaluation of Imatib efflux and influx transporters expression in Chronic Myeloid Leukemia (CML) patients', , 14(1), pp. 49-57.
Solali, Saeid, Kaviani, Saeid, Movassaghpuor, Ali Akbar, Ali-parasti, Mohammad Reza, Soleimani, Masoud, Noruzinia, Mehrdad, Abroun, Saeid, Kaviani, Zeinab Quantitative evaluation of Imatib efflux and influx transporters expression in Chronic Myeloid Leukemia (CML) patients. , 2011; 14(1): 49-57.

Quantitative evaluation of Imatib efflux and influx transporters expression in Chronic Myeloid Leukemia (CML) patients

Pathobiology Reserach
Article 5, Volume 14, Issue 1, 2011, Pages 49-57    PDF (315.86 K)
Authors
Saeid Solali1; Saeid Kaviani* 2; Ali Akbar Movassaghpuor3; Mohammad Reza Ali-parasti4; Masoud Soleimani5; Mehrdad Noruzinia5; Saeid Abroun5; Zeinab Kaviani6
1Department of Hematology, Faculty of Medical Science, Tarbiat Modares University, Tehran, Iran
2Assistant Professor, Department of Hematology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran P.O.Code: 1411713116
3Assistant Professor, Hematology and Oncology Research Center, Tabriz University of Medical Science, Tabriz, Iran
4Department of Immunology, Faculty of Medical Science, Tabriz University of Medical Science, Tabriz, Iran
5Assistant Professor, Department of Hematology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
6M.D. Student, Faculty of Medicine, Tehran University of Medical Sciences, Tehran, Iran
Abstract
Objective: In this study quantitative expression of MDR1 and hOCT1 genes in CML patients and normal people were measured using Real-Time PCR.
Materials and Methods: To study quantitative expression of these genes by real-time PCR, master-mix with syber green was used. Peripheral blood samples from 30 CML patients and 27 normal persons were harvested. Real-time PCR results were analyzed with relative quantification method.
Result: This study showed that in the patients group who were under treatment with Imatib, MDR1 gene expression was increased which was statistically significant. This increase has a direct relation with disease progress. Gene expression in AP and BP patients was also higher than CP patients. In contrast, hOCT1 expression in patients group in comparison with normal group was not statistically significant.
Conclusion: MDR1 increase in leukemic cell membrane results in the reduction of intra-cellular drug concentration. Thus, optimal concentration of drug for inhibition of BCR-ABL tyrosine kinase is not achieved which culminated in disease progression to AP and BP phases. Moreover changes in hOCT1 gene expression as an influx transporter of Imatib could affect intracellular concentration of drug and finally determine therapy outcome. However, in this study hOCT1 gene expression was variable and was not statistically significant.
Keywords
Real-time PCR; MDR1; hOCT1; CML; Gene expression
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