Dadashpour, Mehdi, Rasooli, Iraj, Sorouri Zanjani, Rahim, Sefidkon, Fatemeh, Taghizadeh, Massoud, Darvish Alipour Astaneh, Shakiba (2011). Antimicrobial, nitric oxide radical scavenging and cytotoxic properties of Thymus daenensis essential oil. , 14(1), 37-47.
Mehdi Dadashpour; Iraj Rasooli; Rahim Sorouri Zanjani; Fatemeh Sefidkon; Massoud Taghizadeh; Shakiba Darvish Alipour Astaneh. "Antimicrobial, nitric oxide radical scavenging and cytotoxic properties of Thymus daenensis essential oil". , 14, 1, 2011, 37-47.
Dadashpour, Mehdi, Rasooli, Iraj, Sorouri Zanjani, Rahim, Sefidkon, Fatemeh, Taghizadeh, Massoud, Darvish Alipour Astaneh, Shakiba (2011). 'Antimicrobial, nitric oxide radical scavenging and cytotoxic properties of Thymus daenensis essential oil', , 14(1), pp. 37-47.
Dadashpour, Mehdi, Rasooli, Iraj, Sorouri Zanjani, Rahim, Sefidkon, Fatemeh, Taghizadeh, Massoud, Darvish Alipour Astaneh, Shakiba Antimicrobial, nitric oxide radical scavenging and cytotoxic properties of Thymus daenensis essential oil. , 2011; 14(1): 37-47.

Antimicrobial, nitric oxide radical scavenging and cytotoxic properties of Thymus daenensis essential oil

Pathobiology Reserach
Article 4, Volume 14, Issue 1, 2011, Pages 37-47    PDF (561.57 K)
Authors
Mehdi Dadashpour1; Iraj Rasooli* 2; Rahim Sorouri Zanjani3; Fatemeh Sefidkon4; Massoud Taghizadeh5; Shakiba Darvish Alipour Astaneh6
1Department of Biotechnology, Faculty of Basic Sciences, Shahed University, Tehran, Iran
2Professor, Department of Biology, Faculty of Basic Sciences, Shahed University, Tehran, Iran, P.O.Code: 3319118651
3Associated Professor, Department of Microbiology, Faculty of Medicine, Baghiyatollah University of Medical Sciences, Tehran, Iran
4Professor, Department of Chemistry, Research Institute of Forests and Rangelands, Tehran, Iran
5Assistant Professor, Department of Chemistry, Faculty of Basic Sciences, Shahed University, Tehran-Iran
6Department of Microbiology, Faculty of Basic Sciences, Shahed University, Tehran, Iran
Abstract
Objective: Despite toxic effects of some essential oils, their use is not under control. With a view to increasing trend of utilisation of herbal products, some biological aspects of Thymus daenensis are repoted here for the first time.
Materials and Methods: Antimicrobial properties using disk diffusion and dilution tests, nitric oxide radical scavenging by Marcocci et al method and cytotoxic properties employing dimethylthiazolyl diphenyltetrazolium bromide reduction test were carried out with Thymus daenensis and commercial Thyme essential oils and their main chemical compound, thymol.
Results: The microbial sensitivity to the oils were in Candida albicans> E.coli> S.aureus> P. aeruginosa order. The minimum inhibitory and microbicidal concentrations were in the range of 0.04-10mg/ml. Nitric oxide radical scavenging was dose dependent with an IC50 of 5, 75, 863 µg, and total phenolics of 644.07±6.79, 16.94±2.55, 10.33±2.31µg Gallic acid equivalent per mg sample and total flavonoid content of 73.51±1.34, 0.56±0.02, 0.21±0.09 mg Catechin equivalent per gram T.daenensis oil, commercial thyme oil and thymol respectively. The concentrations from T.daenensis oil, commercial thyme oil and thymol required to exert 50% fatal effect (IC50) on healthy human normal lymphocytes and Hela cells were 1455, 12.10, 2867 and 4.95, 3.61, 1730 µg respectively.
Conclusion: T. daenensis with its good antimicrobial property can prevent formation of toxic reactive oxygen species and as a good antioxidant, it can directly scavenge NO and O2−. With a view to cancerous cells killing properties of the oils at their lowest concentrations without fatal effect on normal healthy cells, feasibility of their application in combating cancerous cells may be promising.
Keywords
Antioxidant; Essential oils; Thymus daenensis; Antimicrobial; Nitricoxide
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