Rabbani, Mohsen, Tafazoli Shadpour, Mohammad, Shokrgozar, Mohammad Ali, پیروی, حبیب اله, Janmaleki, Mohsen, Amirizade, Naser, Haghighipour, Nooshin (2011). Effects of combined cyclic-static stretch on orientation and proliferation of human mesenchymal stem cells. , 13(4), -.
Mohsen Rabbani; Mohammad Tafazoli Shadpour; Mohammad Ali Shokrgozar; حبیب اله پیروی; Mohsen Janmaleki; Naser Amirizade; Nooshin Haghighipour. "Effects of combined cyclic-static stretch on orientation and proliferation of human mesenchymal stem cells". , 13, 4, 2011, -.
Rabbani, Mohsen, Tafazoli Shadpour, Mohammad, Shokrgozar, Mohammad Ali, پیروی, حبیب اله, Janmaleki, Mohsen, Amirizade, Naser, Haghighipour, Nooshin (2011). 'Effects of combined cyclic-static stretch on orientation and proliferation of human mesenchymal stem cells', , 13(4), pp. -.
Rabbani, Mohsen, Tafazoli Shadpour, Mohammad, Shokrgozar, Mohammad Ali, پیروی, حبیب اله, Janmaleki, Mohsen, Amirizade, Naser, Haghighipour, Nooshin Effects of combined cyclic-static stretch on orientation and proliferation of human mesenchymal stem cells. , 2011; 13(4): -.

Effects of combined cyclic-static stretch on orientation and proliferation of human mesenchymal stem cells

Pathobiology Reserach
Article 7, Volume 13, Issue 4, 2011    PDF (2.06 M)
Authors
Mohsen Rabbani; Mohammad Tafazoli Shadpour* 1; Mohammad Ali Shokrgozar2; حبیب اله پیروی; Mohsen Janmaleki3; Naser Amirizade4; Nooshin Haghighipour2
1Department of Biomechanics, Faculty of Biomedical Engineering, Amirkabir University of Technology, Tehran
2National Cell Bank of Iran, Pasteur Institute of Iran, Tehran
3Nanomedicine and Tissue Engineering Research Center, Taleghani Hospital, Parvaneh St., Velenjak
4Assistant Professor, Department of Hematology, Research Center of Iranian Blood Transfusion Organization, Tehran, Iran
Abstract
Objective: Cell vital function has correlation with mechanical loadings that cell experiences. Here, effects of in-vitro combined cyclic-static stretch on proliferation of human mesenchymal stem cell (HMSC) were evaluated.
Materials and Methods: HMSCs were cultured on gelatin coated elastic membranes, and exposed to stretch loading. Four different regimes of cyclic, static, combined cyclic-static, and cyclic with a period of unloading were exerted on the elastic membrane. Duration of cyclic loading and static loading was 5 and 12 hours respectively.
Results: The results illustrate that 10% cyclic stretch causes cell alignment but there were no significant proliferation differences between control and test group. Combined cyclic-static stretch reduced proliferation significantly while cyclic stretch with an unloading period increased cell proliferation significantly. At last, static stretch did not affect cell proliferation significantly.
Conclusion: Cell stretching regimes and post-loading duration are effective factors on cell proliferation.
Keywords
Tissue engineering; Static Stretch; Cyclic Stretch; Cell Proliferation; Human Mesenchymal Stem Cell
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