Ayoubian, Nader, Foroutan, Tahereh, Jamshidifar, Azin, Mosaffa, Nariman (2011). The study of cell viability and alkaline phosphatase activity of saos-2 cells contact with osteon and cerasoeb in vitro. , 13(4), -.
Nader Ayoubian; Tahereh Foroutan; Azin Jamshidifar; Nariman Mosaffa. "The study of cell viability and alkaline phosphatase activity of saos-2 cells contact with osteon and cerasoeb in vitro". , 13, 4, 2011, -.
Ayoubian, Nader, Foroutan, Tahereh, Jamshidifar, Azin, Mosaffa, Nariman (2011). 'The study of cell viability and alkaline phosphatase activity of saos-2 cells contact with osteon and cerasoeb in vitro', , 13(4), pp. -.
Ayoubian, Nader, Foroutan, Tahereh, Jamshidifar, Azin, Mosaffa, Nariman The study of cell viability and alkaline phosphatase activity of saos-2 cells contact with osteon and cerasoeb in vitro. , 2011; 13(4): -.

The study of cell viability and alkaline phosphatase activity of saos-2 cells contact with osteon and cerasoeb in vitro

Pathobiology Reserach
Article 3, Volume 13, Issue 4, 2011    PDF (1.18 M)
Authors
Nader Ayoubian1; Tahereh Foroutan* 2; Azin Jamshidifar1; Nariman Mosaffa3
1Department of Periodontology, Faculty of Dentistry, Islamic Azad University, Tehran Branch
2Department of Biology, Faculty of Basic Science, Tarbiat Moalem University, Tehran
3Professor, Department of Immunology, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
Abstract
Objective: Osteon has been introduced as a bone substitute material. It is biphasic calcium phosphate (BCP) that use in dentistry. The aim of the present study was to evaluate the effect of osteon on the proliferation, cell viability and differentiation of saos-2 cells in vitro. Also it was compared with cerasorb bone graft.
Materials and Methods: Two different bone grafts materials, osteon and cerasorb, were used to evaluate the effect on proliferation and differentiation rate of saos-2 cells. On day 15 the cell proliferation and cell viability was measured by MTT assay. For determination of differentiation, alkaline phosphatase and alizarin red test was used.
Results: Osteon and cerasorb groups showed significantly higher alkaline phosphatase activity and cell differentiation. Cell viability of both bone grafts was significantly lower than control group and cell proliferation was higher in osteon group. Osteon has more suitable biological property compare to cerasorb.
Conclusions: The results of the present study showed that osteon and cersorb bone grafts allow proliferation and differentiation of saos-2 cells in vitro.
Keywords
Bone Substitute Replacement; Cell Viability; Cell Line Saos-2; morphology
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