Norouzi, Roghaye, Dalimi, Abdolhossein, Forouzandeh, Mehdi, Ghaffarifar, Fatemeh (2008). Application of PCR-ELISA method based on RE domain for diagnosis of toxoplasmosis in experimentally infected rat (Rattus norvegicus). , 11(0), -.
Roghaye Norouzi; Abdolhossein Dalimi; Mehdi Forouzandeh; Fatemeh Ghaffarifar. "Application of PCR-ELISA method based on RE domain for diagnosis of toxoplasmosis in experimentally infected rat (Rattus norvegicus)". , 11, 0, 2008, -.
Norouzi, Roghaye, Dalimi, Abdolhossein, Forouzandeh, Mehdi, Ghaffarifar, Fatemeh (2008). 'Application of PCR-ELISA method based on RE domain for diagnosis of toxoplasmosis in experimentally infected rat (Rattus norvegicus)', , 11(0), pp. -.
Norouzi, Roghaye, Dalimi, Abdolhossein, Forouzandeh, Mehdi, Ghaffarifar, Fatemeh Application of PCR-ELISA method based on RE domain for diagnosis of toxoplasmosis in experimentally infected rat (Rattus norvegicus). , 2008; 11(0): -.

Application of PCR-ELISA method based on RE domain for diagnosis of toxoplasmosis in experimentally infected rat (Rattus norvegicus)

Pathobiology Reserach
Article 5, Volume 11, Issue 0, 2008    PDF (388.74 K)
Authors
Roghaye Norouzi1; Abdolhossein Dalimi2; Mehdi Forouzandeh3; Fatemeh Ghaffarifar* 4
1M.Sc. Student, Department of Medical Parasitology, School of Medical Sciences, Tarbiat Modares University, Tehran, Iran
2Professor, Department of Medical Parasitology, School of Medical Sciences, Tarbiat Modares University, Tehran, Iran
3Associated Professor, Department of Medial Biotechnology, School of Medical Sciences, Tarbiat Modares University, Tehran, Iran
4Associated Professor, Department of Medical Parasitology, School of Medical Sciences, Tarbiat Modares University, Tehran, Iran
Abstract
Objective: Toxoplasmosis may cause significant damage to the developing fetus and is a life-threatening opportunistic infection in immunocompromised persons. Molecular methods are known to be more sensitive and more specific than serological assays for diagnosis of toxoplasmosis. Application of quantitative PCR has evolved sensitive, specific, and rapid method for the detection of RH strain of Toxoplasma gondii DNA.
Materials and Methods: In the present study, quantitative PCR-ELISA (Polymerase Chain Reaction-enzyme linked immunosorbent assay) was used for quantization of Toxoplasma gondii in the blood of 15 rats (Rattus norvegicus) infected experimentally with the parasite.
In this regard Polymerase PCR-ELISA was developed for rapid detection of Toxoplasma gondii. Specific primers targeting RE gene were selected and used for the amplification of toxoplasmic DNA. DIG-labeled (digoxigenin-labeled) amplicons were hybridized with a specific biotinylated oligonucleotide probe in solution phase and subsequently transferred to streptavidin coated plates. The captured DNA-DNA hybrids were colorimetrically detected by the addition of anti-digoxigenin antibody peroxidase conjugate and substrate.
Results: DNA of Toxoplasma gondii were efficiently detected within 4 hours and no interference was encountered in the amplification and detection of the parasite.
Conclusion: Efficiency of the PCR-ELISA system was evaluated we found with several advantages in terms of sensitivity, rapidity and simplicity in this system.
Keywords
PCR-ELISA
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