Pouide, Elham, Arefian, Ehsan, Deldar, Ali Asghar, Akhavan sepahy, Abbas (2016). Evaluation of Human Papilloma Virus (HPV) genotypes diversity in oral cancer lesions of patients in Imam Khomeini Hospital. , 19(2), 19-27.
Elham Pouide; Ehsan Arefian; Ali Asghar Deldar; Abbas Akhavan sepahy. "Evaluation of Human Papilloma Virus (HPV) genotypes diversity in oral cancer lesions of patients in Imam Khomeini Hospital". , 19, 2, 2016, 19-27.
Pouide, Elham, Arefian, Ehsan, Deldar, Ali Asghar, Akhavan sepahy, Abbas (2016). 'Evaluation of Human Papilloma Virus (HPV) genotypes diversity in oral cancer lesions of patients in Imam Khomeini Hospital', , 19(2), pp. 19-27.
Pouide, Elham, Arefian, Ehsan, Deldar, Ali Asghar, Akhavan sepahy, Abbas Evaluation of Human Papilloma Virus (HPV) genotypes diversity in oral cancer lesions of patients in Imam Khomeini Hospital. , 2016; 19(2): 19-27.

Evaluation of Human Papilloma Virus (HPV) genotypes diversity in oral cancer lesions of patients in Imam Khomeini Hospital

Pathobiology Reserach
Article 2, Volume 19, Issue 2, 2016, Pages 19-27    PDF (612.61 K)
Authors
Elham Pouide1; Ehsan Arefian* 2; Ali Asghar Deldar3; Abbas Akhavan sepahy4
1Department of Microbiology, Olum Tahghyghat university, Tehran, Iran
2Department of virology, Biology College, Tehran University, Tehran, Iran
3Department of Genetic, Maleke Ashtar University, Tehran, Iran
4Department of Microbiology, Biology College, Azad university, North of Tehran, Tehran, Iran
Abstract
Introduction: Squamous cell carcinoma comprises approximately 94% of all oral cavities. One reasons for this cancer is Human Papilloma Virus (HPV) with different genotypes .Finding the most common genotypes will be helpful to control and prevent of spreading this cancer.
Materials and Method: 70 Paraffinated blocks were collected from cancer department of Imam Khomeini hospital in Tehran. All of these samples included histopathological report of dysplastic lesions .They weredeparaffinated.4 primers were designed for PCR .Then they were transferred to electrophoresis tank. Positive samples were sequenced by Mega 5.
Results: 8 HPV+ samples include of 3 HPV+6 and HPV+16 were found . HPV6 is the cause of genital warts that can spread by skin contact or oral- sexual behavior. 3 positive samples were found in women and the others were in men. (2% more in men) . People between 30 to45 are more sensitive for HPV than the other group. People up to 60 years old are sensitive too. All the samples were collected from different cities of Iran but most of the positive samples were found in Tehran and Islam Shahr.
Conclusion: These data confirm that HPV infection with high risk types (6, 16) could be one of the risk factors for oral cancer and it can spread by genital warts.
Keywords
"Human papilloma virus"; "HPV-6"; "HPV-16"; "squamous cell Carcinoma"; "Polymerase Chain Reaction (PCR)"
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