Masti, Yousef, Saadsti, Mojtaba, Nazarian, Shahram, Fasihi Ramandi, Mahdi, Asadi, Morteza, Arefpour, Mohammadali (2015). Production of Antibody against CtxB Recombinant Protein based on Nanofibers and Nanocapsules. , 17(4), 53-61.
Yousef Masti; Mojtaba Saadsti; Shahram Nazarian; Mahdi Fasihi Ramandi; Morteza Asadi; Mohammadali Arefpour. "Production of Antibody against CtxB Recombinant Protein based on Nanofibers and Nanocapsules". , 17, 4, 2015, 53-61.
Masti, Yousef, Saadsti, Mojtaba, Nazarian, Shahram, Fasihi Ramandi, Mahdi, Asadi, Morteza, Arefpour, Mohammadali (2015). 'Production of Antibody against CtxB Recombinant Protein based on Nanofibers and Nanocapsules', , 17(4), pp. 53-61.
Masti, Yousef, Saadsti, Mojtaba, Nazarian, Shahram, Fasihi Ramandi, Mahdi, Asadi, Morteza, Arefpour, Mohammadali Production of Antibody against CtxB Recombinant Protein based on Nanofibers and Nanocapsules. , 2015; 17(4): 53-61.

Production of Antibody against CtxB Recombinant Protein based on Nanofibers and Nanocapsules

Pathobiology Reserach
Article 5, Volume 17, Issue 4, 2015, Pages 53-61    PDF (747.7 K)
Authors
Yousef Masti1; Mojtaba Saadsti* 1; Shahram Nazarian1; Mahdi Fasihi Ramandi2; Morteza Asadi1; Mohammadali Arefpour1
1Department of Biological Sciences, Faculty of Basic Sciences, Imam Hossein University, Tehran, Iran
2Molecular biology research center, Baqiyatallah university of medical sciences, Tehran, Iran
Abstract
Objectives: CtxB (Cholera toxin B subunit) contributes to a vaccine's efficacy by stimulating production of the anti-CtxB antibody. Various attempts have been made to increase production of this antibody. Chitosan is a mucoadhesive polysaccharide that has tremendous potential for oral vaccine delivery in terms of its exclusive features that include biocompatibility, biodegradability, high charge density and non-toxicity. We investigated the potential for chitosan nano­fibers and nanocapsules as novel carrier systems for the oral delivery of CtxB.
Methods: Antigen-containing chitosan nanofibers were prepared by electrospinning a chitosan/AcOH solution. Encapsulation of the antigen inside the chitosan nanofibers was confirmed through infrared spectroscopy analysis (FTIR). Guinea pigs were immunized with free antigen and CtxB antigen or antigen alone by direct administration of antigen-containing chitosan nanofibers into the buccal cavity. Serum immunoglobulin G (IgG) and intestinal immunoglobulin A (IgA) antibody responses were determined
Results: The results indicated that antigen in the chitosan nanofibers or nanocapsules elicited very high IgA and IgG responses. No detectable IgA and IgG responses were obtained after oral immuniza­tion with CtxB. The results of the antibody titer were analyzed using the ANOVA and LSD tests.
Conclusion: CtxB inside the nanofiber increased antibody production when administered orally. This system might be used for delivery of other antigens.
Keywords
CtxB; Chitosan; nanocapsule; Nanofiber; Antibody Titer
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