Sadeghizadeh, Majid, Ebrahimi, Saeideh, Sadeghizadeh, Majid, Aghasadeghi, Mohammad, Shafiee Ardestani, Mehdi, Behmanesh, Mehrdad, Behmanesh, Mehrdad (2015). One-step Synthesis of Anionic PEG-citrate G2 Dendrimer and Evaluation of its Cytotoxicity in Two Cell Lines. , 17(4), 13-24.
Majid Sadeghizadeh; Saeideh Ebrahimi; Majid Sadeghizadeh; Mohammad Aghasadeghi; Mehdi Shafiee Ardestani; Mehrdad Behmanesh; Mehrdad Behmanesh. "One-step Synthesis of Anionic PEG-citrate G2 Dendrimer and Evaluation of its Cytotoxicity in Two Cell Lines". , 17, 4, 2015, 13-24.
Sadeghizadeh, Majid, Ebrahimi, Saeideh, Sadeghizadeh, Majid, Aghasadeghi, Mohammad, Shafiee Ardestani, Mehdi, Behmanesh, Mehrdad, Behmanesh, Mehrdad (2015). 'One-step Synthesis of Anionic PEG-citrate G2 Dendrimer and Evaluation of its Cytotoxicity in Two Cell Lines', , 17(4), pp. 13-24.
Sadeghizadeh, Majid, Ebrahimi, Saeideh, Sadeghizadeh, Majid, Aghasadeghi, Mohammad, Shafiee Ardestani, Mehdi, Behmanesh, Mehrdad, Behmanesh, Mehrdad One-step Synthesis of Anionic PEG-citrate G2 Dendrimer and Evaluation of its Cytotoxicity in Two Cell Lines. , 2015; 17(4): 13-24.

One-step Synthesis of Anionic PEG-citrate G2 Dendrimer and Evaluation of its Cytotoxicity in Two Cell Lines

Pathobiology Reserach
Article 2, Volume 17, Issue 4, 2015, Pages 13-24    PDF (991.59 K)
Authors
Majid Sadeghizadeh* 1; Saeideh Ebrahimi2; Majid Sadeghizadeh* 1; Mohammad Aghasadeghi3; Mehdi Shafiee Ardestani4; Mehrdad Behmanesh1; Mehrdad Behmanesh1
1Department of Molecular Genetics, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran
2Department of Nanobiotechnology, Faculty of Biological Sciences, Tarbiat Modares University,Tehran, Iran
3Department of Hepatitis and AIDS, Pasteur Institute of Iran, Tehran, Iran
4Department of pharmacy, Tehran University of Medical Sciences, Tehran, Iran
Abstract
Objective: Dendrimers are three-dimensional nanostructures that have numerous applications in medicine, including drug delivery and imaging. Although anionic dendrimer polyethylene glycol–citrate has a high potential to increase solubility of water-insoluble drugs and drug delivery, its multi-step synthesis procedure is time consuming. In addition, toxic substances such as dichloromethane are used in its synthesis procedure. In this study, we have developed a simple one-step synthesis method using green chemistry.
Methods: We examined four different methods to improve the synthesis method of this dendrimer. Products were characterized by FTIR, LC-MS and DLS. Cytotoxicity was assessed by the XTT method.
Results: We synthesized a G2 polyethylene glycol–citrate dendrimer in one-step without purifying G1. This process was chosen as a beneficial method for synthesis of the G2 dendrimer. When compared with previous methods, this procedure had higher efficiency and greatly reduced response. This procedure used nontoxic materials. XTT assay results showed that this dendrimer created by green chemistry had no cytotoxicity in Hela and Vero cells up to a concentration of 800 µM.
Conclusion: One-step synthesis of anionic polyethylene glycol-citrate G2 dendrimer is a simple, beneficial production method. The dendrimer is biocompatible and can be used as a suitable carrier for drug delivery purposes.
Keywords
Biodegradable; Improvement; Dendrimer; Green Chemistry; PEG-Citric Acid
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