Hadjati, Jamshid, آماری, افشین, Razavi, AliReza, Jamali, Arezoo, Amini Sardrod, AbbasAli, Motamedi, Masomeh, Shojaee, Saeedeh, Ansaripour, Bita (2009). Effect of protein and DNA components of Toxoplasma gondii on IL-12 production from dendritic cells and T cell proliferation. , 12(3), 1-8.
Jamshid Hadjati; افشین آماری; AliReza Razavi; Arezoo Jamali; AbbasAli Amini Sardrod; Masomeh Motamedi; Saeedeh Shojaee; Bita Ansaripour. "Effect of protein and DNA components of Toxoplasma gondii on IL-12 production from dendritic cells and T cell proliferation". , 12, 3, 2009, 1-8.
Hadjati, Jamshid, آماری, افشین, Razavi, AliReza, Jamali, Arezoo, Amini Sardrod, AbbasAli, Motamedi, Masomeh, Shojaee, Saeedeh, Ansaripour, Bita (2009). 'Effect of protein and DNA components of Toxoplasma gondii on IL-12 production from dendritic cells and T cell proliferation', , 12(3), pp. 1-8.
Hadjati, Jamshid, آماری, افشین, Razavi, AliReza, Jamali, Arezoo, Amini Sardrod, AbbasAli, Motamedi, Masomeh, Shojaee, Saeedeh, Ansaripour, Bita Effect of protein and DNA components of Toxoplasma gondii on IL-12 production from dendritic cells and T cell proliferation. , 2009; 12(3): 1-8.

Effect of protein and DNA components of Toxoplasma gondii on IL-12 production from dendritic cells and T cell proliferation

Pathobiology Reserach
Article 1, Volume 12, Issue 3, 2009, Pages 1-8    PDF (257.04 K)
Authors
Jamshid Hadjati* 1; افشین آماری; AliReza Razavi2; Arezoo Jamali3; AbbasAli Amini Sardrod4; Masomeh Motamedi2; Saeedeh Shojaee5; Bita Ansaripour2
1Department of Immunology, Faculty of Medicine, Tehran University of Medical Sciences, Tehran, Iran
2Department of Immunology, Faculty of Public Health, Tehran University of Medical Sciences, Tehran, Iran
3Department of Immunology, Faculty of Paramedicene , Tehran University of Medical Sciences, Tehran, Iran
4Department of Immunology, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
5Department of Parasithology, Faculty of Public Health, Tehran University of Medical Sciences, Tehran, Iran
Abstract
Objective: The aim of the present study was to investigate the effect of protein and DNA components of Toxoplasma gondii on maturation of dendritic cells and their efficiency in IL-12 production and proliferation of T cells.
Materials and Methods: for DC generation, Bone marrow cells were cultured in the presence of GM-CSF and IL-4 for 5 days. Tumor lysate and protein or DNA components of Toxoplasma gondii were added to the culture media and incubated for another 2 days. LPS was added as control for DC maturation. Proliferation of T cells were determined by MLR and IL-12 production was measured by ELISA kit. Maturation of dendritic cell were determined by flowcytometry.
Results: DCs treatment with protein components of Toxoplasma gondii caused a significant increase in IL-12 production and proliferation of T cells (P<0.001).
Conclusions: Different compositions of microbial body like protein and DNA components of Toxoplasma gondii can cause augmentation of antigen presentation capacity of DC and their IL-12 production capability. Among these components the protein was more effective as compared to DNA.
Keywords
IL-12; Dendritic cells; Toxoplasma gondii
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