Vazifeh Shiran, Nader, Soleimani, Masoud, Mortazavi, Yousef, Kaviani, Saeed, Aboulghasemi, Hassan, Nikougoftar, Mahin (2010). 3D culture of umblical cord blood-drived CD34+ cells on a DBM scaffold coated by cord blood–derived unrestricted somatic stem cells (USSC). , 13(3), 63-81.
Nader Vazifeh Shiran; Masoud Soleimani; Yousef Mortazavi; Saeed Kaviani; Hassan Aboulghasemi; Mahin Nikougoftar. "3D culture of umblical cord blood-drived CD34+ cells on a DBM scaffold coated by cord blood–derived unrestricted somatic stem cells (USSC)". , 13, 3, 2010, 63-81.
Vazifeh Shiran, Nader, Soleimani, Masoud, Mortazavi, Yousef, Kaviani, Saeed, Aboulghasemi, Hassan, Nikougoftar, Mahin (2010). '3D culture of umblical cord blood-drived CD34+ cells on a DBM scaffold coated by cord blood–derived unrestricted somatic stem cells (USSC)', , 13(3), pp. 63-81.
Vazifeh Shiran, Nader, Soleimani, Masoud, Mortazavi, Yousef, Kaviani, Saeed, Aboulghasemi, Hassan, Nikougoftar, Mahin 3D culture of umblical cord blood-drived CD34+ cells on a DBM scaffold coated by cord blood–derived unrestricted somatic stem cells (USSC). , 2010; 13(3): 63-81.

3D culture of umblical cord blood-drived CD34+ cells on a DBM scaffold coated by cord blood–derived unrestricted somatic stem cells (USSC)

Pathobiology Reserach
Article 7, Volume 13, Issue 3, 2010, Pages 63-81    PDF (5.45 M)
Authors
Nader Vazifeh Shiran1; Masoud Soleimani* 1; Yousef Mortazavi2; Saeed Kaviani1; Hassan Aboulghasemi3; Mahin Nikougoftar3
1Department of Hematology and Blood Banking, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
2Associated Professor, Department of Pathology, Faculty of Medicine, Zanjan University of Medical Sciences
3Research Center of Iranian Blood Transfusion Organization, Tehran, Iran
Abstract
Objective: Allogeneic transplantation with umbilical cord blood (UCB) in adult recipients is limited mainly by a low CD34+ cell dose. To overcome this shortcoming, human placenta as a novel source of human mesenchymal progenitor cell (MPC)- unrestricted somatic stem cells (USSC)- was incorporated in an attempt to expand CD34+ cells from UCB. To provide a similar environment in vitro, we coated DBM scaffold with USSC cells as the matrix for support UCB-CD34+ cells growth.
Materials and Methods: Human placenta USSC was isolated and characterized by morphologic and immunophenotypical analysis. UCB CD34+ cells were expanded by coculture with placental USSC in 2D and 3D environment. Suitable aliquots of cells were used to monitor cell production, clonogenic activity, and long-term culture-initiating culture (LTC-IC) output.
Results: Ex vivo expansion of UCB hematopoietic cells, when cultured in different 2D conditions and 3D condition for 3 weeks, was significantly enhanced, the total cell count increased within the 28-day period. For total CFC, the highest CFC expansion was observed at day 14. Flow cytometry analysis of the percentage of CD34+ cells showed a decline in USSC cocultures in 2D and 3D condition at 3 weeks.
Conclusion: These results strongly suggest that human USSC may be a suitable feeder layer for expansion of hematopoietic progenitors from UCB in vitro and USSC- coated DBM can therefore provide an ex vivo mimicry of bone marrow by enhancing of surface/ volume ratio and feeder layers,recapitulate the desired niche, and provide a suitable environment for stem cell expansion and differentiation.
Keywords
UCB-CD34+ Cells; Expansion; USSC; 3D Scaffold; DBM
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