Saki, Najmeddin, Abroun, Saeid, Soleimani, Masoud, Kaviani, Saeid Kaviani, Sadeghi, Bahare, Vaghef, Najme (2010). The role of myeloma cells in the in-vitro diffrentiation of cord blood hematopoietic stem cell to osteoclast. , 13(3), 53-61.
Najmeddin Saki; Saeid Abroun; Masoud Soleimani; Saeid Kaviani Kaviani; Bahare Sadeghi; Najme Vaghef. "The role of myeloma cells in the in-vitro diffrentiation of cord blood hematopoietic stem cell to osteoclast". , 13, 3, 2010, 53-61.
Saki, Najmeddin, Abroun, Saeid, Soleimani, Masoud, Kaviani, Saeid Kaviani, Sadeghi, Bahare, Vaghef, Najme (2010). 'The role of myeloma cells in the in-vitro diffrentiation of cord blood hematopoietic stem cell to osteoclast', , 13(3), pp. 53-61.
Saki, Najmeddin, Abroun, Saeid, Soleimani, Masoud, Kaviani, Saeid Kaviani, Sadeghi, Bahare, Vaghef, Najme The role of myeloma cells in the in-vitro diffrentiation of cord blood hematopoietic stem cell to osteoclast. , 2010; 13(3): 53-61.

The role of myeloma cells in the in-vitro diffrentiation of cord blood hematopoietic stem cell to osteoclast

Pathobiology Reserach
Article 6, Volume 13, Issue 3, 2010, Pages 53-61    PDF (2.04 M)
Authors
Najmeddin Saki1; Saeid Abroun* 2; Masoud Soleimani1; Saeid Kaviani Kaviani1; Bahare Sadeghi1; Najme Vaghef1
1Department of Hematology, School of Medical Sciences, Tarbiat Modares University Tehran, Iran
2Assistant Professor, Department of Hematology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
Abstract
Objective: Average Age of population in the industrial countries has increased. Because of aging the percent of the diseases related to the oldness such as multiple myeloma have also increased. It has both common and unique symptoms and effects. The unique effects include wide bone reabsorption. It seems necessary to understand the structure of Bone Marrow Niche and the effects of Myeloma cells on adjacent hematopoietic Stem cells with a new approach.
Materials and Methods: We have studied the differentiating effect induced by the Myeloma cells through co-culturing the Myeloma cells and hematopoietic stem cells, extracted out of cord blood. In this investigation we also cocultured myeloma cells with the monoblastic cell line (U937) in order to evaluate the effect of myeloma cells on monoblastic cells differentiation.
Results: Our findings show that increased expression of myeloid and monocytoid markers in coculturing of myeloma cells and HSCs. Moreover following monoblastic and myeloid cells coculturing, we observed probably TRAP positive osteoclastic like cells.
Conclusion: Our findings show that presence of myeloma cells in Bone Marrow play essential role in HSCs differentiation to monocytoid (osteoclastic) lineage.
Keywords
Niche; Multiple Myeloma; Hematopoietic Stem Cells; Osteoclast Differentiation
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