Antifungal Activity of Aureobasidin A in Combination with Fluconazole against Fluconazole-Resistant Candida albicans | ||
| Infection Epidemiology and Microbiology | ||
| Article 5, Volume 6, Issue 4, 2020, Pages 285-291 PDF (351.37 K) | ||
| Document Type: Original Research | ||
| DOI: 10.29252/iem.6.4.285 | ||
| Authors | ||
| Shadi Alimehr1; Masoomeh Shams-Ghahfarokhi* 1; Mehdi Razzaghi Abyaneh2 | ||
| 1Department of Mycology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran 14115-331, Iran | ||
| 2Department of Mycology, Pasteur Institute of Iran, Tehran 13164, Iran | ||
| Abstract | ||
| Background: Aureobasidin A is known as a cyclic depsipeptide antibiotic with toxic effects against yeasts such as Candida spp at low concentration. Combination therapy is used as a conventional treatment for fungal infections, especially drug-resistant cases. The current study aimed to investigate the combined effects of fluconazole and Aureobasidin A on fluconazole-resistant C. albicans isolates using broth microdilution method. Materials & Methods: Antifungal activity of Aureobasidin A (AbA) compared to fluconazole against C. albicans ATCC 76615 strain was determined using the standardized broth microdilution method according to the Clinical and Laboratory Standards Institute (CLSI, document M27-Ed4) guidelines. The checkerboard method was used to test the combined effects of Aureobasidin A and fluconazole. The synergy, indifference, and antagonism were defined based on the fractional inhibitory concentration values below 0.5, 0.5-4, and more than 4 μg/mL, respectively. Findings: MIC50 and MIC90 evaluations of Aureobasidin A and fluconazole were done at concentrations of 0.25-2 and 32-64 μg/mL against C. glabrata isolates, respectively. The synergy between fluconazole and Aureobasidin A was observed against Candida isolate. A reduced MIC was demonstrated against C. albicans isolate when fluconazole was combined with Aureobasidin A at 4 to 0.12 μg/mL concentrations. Conclusion: The present study findings revealed that Aureobasidin A combined with fluconazole exhibited potent inhibitory effects against fluconazole-resistant C. albicans isolates. Further studies is recommended to investigate the synergistic effects of Aureobasidin A and other antifungal drugs. | ||
| Keywords | ||
| Minimum inhibitory concentration (MIC); Fluconazole-resistance; Aureobasidin A; Candida albicans | ||
| References | ||
|
| ||
|
Statistics Article View: 144 PDF Download: 92 |
||
| Number of Journals | 45 |
| Number of Issues | 2,171 |
| Number of Articles | 24,674 |
| Article View | 24,436,255 |
| PDF Download | 17,551,354 |