Beheshti, N., Ghaffari Far, F., Sharifi, Z., Eslamirad, Z., Farivar Sadr, M., Dayer, M.S., Nasiri, V., Ebrahimisadr, P., Hamidianfar, N., Bayesh, S., Jorjani, O. (2018). Phylogenetic Evaluation and Molecular Variation of Leishmania major and Leishmania tropica Isolated from Different Parts of Iran. , 4(4), 139-145.
N. Beheshti; F. Ghaffari Far; Z. Sharifi; Z. Eslamirad; M. Farivar Sadr; M.S. Dayer; V. Nasiri; P. Ebrahimisadr; N. Hamidianfar; S. Bayesh; O. Jorjani. "Phylogenetic Evaluation and Molecular Variation of Leishmania major and Leishmania tropica Isolated from Different Parts of Iran". , 4, 4, 2018, 139-145.
Beheshti, N., Ghaffari Far, F., Sharifi, Z., Eslamirad, Z., Farivar Sadr, M., Dayer, M.S., Nasiri, V., Ebrahimisadr, P., Hamidianfar, N., Bayesh, S., Jorjani, O. (2018). 'Phylogenetic Evaluation and Molecular Variation of Leishmania major and Leishmania tropica Isolated from Different Parts of Iran', , 4(4), pp. 139-145.
Beheshti, N., Ghaffari Far, F., Sharifi, Z., Eslamirad, Z., Farivar Sadr, M., Dayer, M.S., Nasiri, V., Ebrahimisadr, P., Hamidianfar, N., Bayesh, S., Jorjani, O. Phylogenetic Evaluation and Molecular Variation of Leishmania major and Leishmania tropica Isolated from Different Parts of Iran. , 2018; 4(4): 139-145.

Phylogenetic Evaluation and Molecular Variation of Leishmania major and Leishmania tropica Isolated from Different Parts of Iran

Infection Epidemiology and Microbiology
Article 4, Volume 4, Issue 4 - Serial Number 13, 2018, Pages 139-145    PDF (1.63 M)
Document Type: Original Research
Authors
N. Beheshti1; F. Ghaffari Far* 1; Z. Sharifi2; Z. Eslamirad3; M. Farivar Sadr4; M.S. Dayer1; V. Nasiri5; P. Ebrahimisadr1; N. Hamidianfar6; S. Bayesh7; O. Jorjani8
1Parasitology Department, Medical Sciences Faculty, Tarbiat Modares University, Tehran, Iran
2Blood Transfusion Research Center, High Institute for Research & Education in Transfusion Medicine, Tehran, Iran
3Parasitology Department, Medical Sciences Faculty, Arak University of Medical Sciences, Arak, Iran
4Parasitology Department, Medical Sciences Faculty, Tehran University of Medical Sciences, Tehran, Iran
5Parasitology Department, Razi Vaccine & Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Alborz, Iran
6Natural Resources & Environmental Sciences Department, Agriculture & Natural Resources Faculty, Khorasgan (Isfahan) Branch, Islamic Azad University Isfahan, Iran
7Students Research Committee, Tehran Medical Sciences Branch, Islamic Azad Univesity, Tehran, Iran
8Laboratory Science Research Center, Golestan University of Medical Sciences, Gorgan, Iran
Abstract
​Aims: The aim of this study was species identification and analysis of species of Leishmania isolated from clinical samples.

Materials & Methods: The samples were collected from patients that were infected from different parts of Iran. After microscopic examination, we used PCR method for the ITS1 (internal transcribed spacer 1) RFLP method (digestion with and for phylogenetic construction, DNA sequencing of

Findings: Two samples from Khorasan province (Mashhad) were Leishmania (L. ), while others were Leishmania major (L. ). L. more variable compared with L. . The molecular sequencing differences between L. to geographical distribution. Based on the results of PCR product in the gel electrophoresis and DNA sequencing for L. L. , the DNA sizes were between 350 and 369bp. The RFLP for L. L. and one respectively. The sequences all samples from central parts are the same, but there is difference with the samples isolated from of Iran.

Conclusion: The sequences of of Leishmania major separated from Damghan and Esfarayen are different from other samples. Similarity of DNA sequences of North-East part of Iran of L. from central parts was 99%. The similarity of two isolates of L. 96%. The most similarity of Leishmania isolated was 95% with Indian isolate and the most similarity for Leishmania major was 99% with Friedlin strain.
Keywords
Leishmania major; PCR; RFLP; Sequencing; Phylogenetic analysis; Iran
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