Ghods, Nayereh, Falahati, Mehraban, Roudbary, Maryam, Mardani, Sara, Seif, Farhad (2017). Presence of HSP90 Gene in Amphotericin B Resistant Aspergillus Species Isolated from Iranian Immunocompromised Patients. , 3(3), 86-89.
Nayereh Ghods; Mehraban Falahati; Maryam Roudbary; Sara Mardani; Farhad Seif. "Presence of HSP90 Gene in Amphotericin B Resistant Aspergillus Species Isolated from Iranian Immunocompromised Patients". , 3, 3, 2017, 86-89.
Ghods, Nayereh, Falahati, Mehraban, Roudbary, Maryam, Mardani, Sara, Seif, Farhad (2017). 'Presence of HSP90 Gene in Amphotericin B Resistant Aspergillus Species Isolated from Iranian Immunocompromised Patients', , 3(3), pp. 86-89.
Ghods, Nayereh, Falahati, Mehraban, Roudbary, Maryam, Mardani, Sara, Seif, Farhad Presence of HSP90 Gene in Amphotericin B Resistant Aspergillus Species Isolated from Iranian Immunocompromised Patients. , 2017; 3(3): 86-89.

Presence of HSP90 Gene in Amphotericin B Resistant Aspergillus Species Isolated from Iranian Immunocompromised Patients

Infection Epidemiology and Microbiology
Article 4, Volume 3, Issue 3 - Serial Number 8, 2017, Pages 86-89    PDF (401.33 K)
Authors
Nayereh Ghods1; Mehraban Falahati1; Maryam Roudbary* 1; Sara Mardani2; Farhad Seif3
1Department of Medical Mycology and Parasitology, School of Medicine, Iran University of Medical Sciences, Tehran, IR Iran
2Department of Mycology, Faculty of Medical Science, Tarbiat Modares University, Tehran, IR Iran
3Department of Immunology, School of Medicine, Iran University of Medical Sciences, Tehran, IR Iran
Abstract
Background: Aspergillosis is an opportunistic infection caused by Aspergillus spp in immunocompromised patients. The role of HSP90 in Aspergillus drug resistance is still unknown. The aim of this study was to evaluate the correlation between the presence of HSP90 gene and polyene resistance in Aspergillus spp using PCR.
Materials and Methods: In this study, 32 Aspergillus strains were used, which were isolated from patients susceptible to aspergillosis through Bronchoalveolar lavage (BAL) and identified by conventional methods. The isolates were cultured on Sabouraud dextrose agar (SDA). Susceptibility testing against amphotericin B was conducted according CLSI standards (M38-A). Also, the presence of HSP90 gene was evaluated using PCR.
Results: Of 32 Aspergillus strains used in this study, 16 (50%) isolates were identified as A. Flavus, 12 (37.5%) isolates as A. fumigatus, and 4 (12.5%) isolates as A. terreus. Among these species, 19 (59.37%) isolates were sensitive to amphotericin B whereas 13 (40.62%) were resistant. Moreover, there was a significant difference between the presence of HSP90 gene and resistance to amphotericin B in Aspergillus species.

Conclusions: The presence of HSP90 gene provides evidence that shows this gene may play important role in resistance to amphotericin B in Aspergillus isolates. Although numerous regulatory genes are involved in resistance mechanisms, they remaines to be more clarified
Keywords
Aspergillus spp; Amphotericin B; HSP90 gene; PCR
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