Amhis, Wahiba, Baiod-Chorfi, Safa, RAAF, Naïma, Benhassine, Fadhila, Ouar-Korichi, Mounira (2017). Helicobacter pylori in children: Molecular characterization, antibiotic resistance and MLST typing of isolated strains in an Algerian hospital. , 3(3), 73-77.
Wahiba Amhis; Safa Baiod-Chorfi; Naïma RAAF; Fadhila Benhassine; Mounira Ouar-Korichi. "Helicobacter pylori in children: Molecular characterization, antibiotic resistance and MLST typing of isolated strains in an Algerian hospital". , 3, 3, 2017, 73-77.
Amhis, Wahiba, Baiod-Chorfi, Safa, RAAF, Naïma, Benhassine, Fadhila, Ouar-Korichi, Mounira (2017). 'Helicobacter pylori in children: Molecular characterization, antibiotic resistance and MLST typing of isolated strains in an Algerian hospital', , 3(3), pp. 73-77.
Amhis, Wahiba, Baiod-Chorfi, Safa, RAAF, Naïma, Benhassine, Fadhila, Ouar-Korichi, Mounira Helicobacter pylori in children: Molecular characterization, antibiotic resistance and MLST typing of isolated strains in an Algerian hospital. , 2017; 3(3): 73-77.

Helicobacter pylori in children: Molecular characterization, antibiotic resistance and MLST typing of isolated strains in an Algerian hospital

Infection Epidemiology and Microbiology
Article 1, Volume 3, Issue 3 - Serial Number 8, 2017, Pages 73-77    PDF (659.29 K)
Authors
Wahiba Amhis1; Safa Baiod-Chorfi2; Naïma RAAF* 3; Fadhila Benhassine2; Mounira Ouar-Korichi4
1Central Laboratory of Clinical Biology, Ibn Ziri Bologhine Hospital, Algiers, Algeria
2Pediatrics service, Ibn Ziri Bologhine Hospital, Algiers, Algeria
31) Department of Microbiology, Faculty of Nature and Life Sciences, University Ferhat Abbas Setif, Setif, Algeria 2) Central Laboratory of Clinical Biology, Ibn Ziri Bologhine Hospital, Algiers, Algeria
4Laboratory of Enterobacteriaceae and Related Bacteria, Institut Pasteur, Algiers Algeria
Abstract
Background: Helicobacter pylori infection is generally acquired in childhood. Algeria is a country with a high prevalence of H. pylori infection. The aim of this work was to take stock of H. pylori infection in Algerian children.
Materials and Methods: About 31antral biopsies were cultured, and then antibiotic susceptibility testing was performed. The statuses of cagPAI and vacA s, m, I, and d regions were determined as well as geographical typing was done by MultiLocus Sequence Typing (MLST) method.
Results: Culture was H. pylori positive in 12 children. Only one resistance to clarithromycin and one to metronidazole were detected. Four out of six strains possessed cagPAI, and five out of six strains were identified as vacA s2m2i2d2. The five strains tested by MLST were of the hpEurope type.
Conclusion: This study revealed high prevalence of H. pylori infection and low resistance to antibiotics and reported for the first time in Algeria a genetic typing of H. pylori strains isolated from Pediatrics.
Keywords
Culture; Gastric biopsies; Antibiotics resistance; cagPAI; vacA
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