Kalantar, Enayatollah, Madani, Majid, Hatami, Amir, Dehghan, Mohammad Hossein, Ebadi, Monireh, Nazari, Morteza, Saifei, Saber, Amin Marashi, Seyed Mahmoud (2017). Multiplex PCR for Detection of a Successful Pathogen; Acinetobacter baumanni as a Real Threat in Intensive Care Unit of a University Hospital. , 3(1), 23-24.
Enayatollah Kalantar; Majid Madani; Amir Hatami; Mohammad Hossein Dehghan; Monireh Ebadi; Morteza Nazari; Saber Saifei; Seyed Mahmoud Amin Marashi. "Multiplex PCR for Detection of a Successful Pathogen; Acinetobacter baumanni as a Real Threat in Intensive Care Unit of a University Hospital". , 3, 1, 2017, 23-24.
Kalantar, Enayatollah, Madani, Majid, Hatami, Amir, Dehghan, Mohammad Hossein, Ebadi, Monireh, Nazari, Morteza, Saifei, Saber, Amin Marashi, Seyed Mahmoud (2017). 'Multiplex PCR for Detection of a Successful Pathogen; Acinetobacter baumanni as a Real Threat in Intensive Care Unit of a University Hospital', , 3(1), pp. 23-24.
Kalantar, Enayatollah, Madani, Majid, Hatami, Amir, Dehghan, Mohammad Hossein, Ebadi, Monireh, Nazari, Morteza, Saifei, Saber, Amin Marashi, Seyed Mahmoud Multiplex PCR for Detection of a Successful Pathogen; Acinetobacter baumanni as a Real Threat in Intensive Care Unit of a University Hospital. , 2017; 3(1): 23-24.

Multiplex PCR for Detection of a Successful Pathogen; Acinetobacter baumanni as a Real Threat in Intensive Care Unit of a University Hospital

Infection Epidemiology and Microbiology
Article 7, Volume 3, Issue 1 - Serial Number 6, 2017, Pages 23-24    PDF (622.16 K)
Authors
Enayatollah Kalantar1; Majid Madani2; Amir Hatami2; Mohammad Hossein Dehghan3; Monireh Ebadi1; Morteza Nazari2; Saber Saifei2; Seyed Mahmoud Amin Marashi* 1
1Department of Microbiology, School of Medicine, Alborz University of Medical Sciences, Karaj, Iran.
2Diagnostic Laboratory, Shahid Rajaei Hospital, Alborz University of Medical Sciences, Karaj, Iran.
3Department of Biochemistry, School of Medicine, Alborz University of Medical Sciences, Karaj, Iran.
Abstract
Multidrug resistance in Acinetobacter baumannii is a growing public health concern all over the world. In the current study, the isolation and antimicrobial resistance pattern and detection of blaOXA-51 and lpxC genes by multiplex PCR method was performed.
All the isolates demonstrated high levels of resistance rates to amikacin, ciprofloxacin, meropenem, imipenem, ceftriaxone, gentamicin, and colistin. Screening of two resistance genes by multiplex PCR showed that all the isolates contained blaOXA-51 and lpxC genes. As we previously reported, nosocomial infections caused by A. baumannii isolates are a major cause of morbidity and mortality in our hospital.
Keywords
Acinetobacter baumannii; Nosocomial infection; multidrug-resistance; blaOXA-51 and lpxC genes
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