Angoti, Goli, Bandehpour, Mojgan, Goudarzi, Hossein, Hajizadeh, Maryam, Zarringhalam Moghaddam, Maryam, Kouchaki, Amaneh (2016). Detection of Efflux Pump Genes (adeA, adeB, adeC and abeM) in Acinetobacter baumannii Isolated from Hospitalize Patients, North-west of Iran. , 2(4), 8-11.
Goli Angoti; Mojgan Bandehpour; Hossein Goudarzi; Maryam Hajizadeh; Maryam Zarringhalam Moghaddam; Amaneh Kouchaki. "Detection of Efflux Pump Genes (adeA, adeB, adeC and abeM) in Acinetobacter baumannii Isolated from Hospitalize Patients, North-west of Iran". , 2, 4, 2016, 8-11.
Angoti, Goli, Bandehpour, Mojgan, Goudarzi, Hossein, Hajizadeh, Maryam, Zarringhalam Moghaddam, Maryam, Kouchaki, Amaneh (2016). 'Detection of Efflux Pump Genes (adeA, adeB, adeC and abeM) in Acinetobacter baumannii Isolated from Hospitalize Patients, North-west of Iran', , 2(4), pp. 8-11.
Angoti, Goli, Bandehpour, Mojgan, Goudarzi, Hossein, Hajizadeh, Maryam, Zarringhalam Moghaddam, Maryam, Kouchaki, Amaneh Detection of Efflux Pump Genes (adeA, adeB, adeC and abeM) in Acinetobacter baumannii Isolated from Hospitalize Patients, North-west of Iran. , 2016; 2(4): 8-11.

Detection of Efflux Pump Genes (adeA, adeB, adeC and abeM) in Acinetobacter baumannii Isolated from Hospitalize Patients, North-west of Iran

Infection Epidemiology and Microbiology
Article 2, Volume 2, Issue 4 - Serial Number 5, 2016, Pages 8-11    PDF (512.97 K)
Authors
Goli Angoti1; Mojgan Bandehpour2; Hossein Goudarzi* 3; Maryam Hajizadeh4; Maryam Zarringhalam Moghaddam1; Amaneh Kouchaki2
1Department of Microbiology, Shahid Beheshti University, M.C International Branch, Tehran, IR Iran
2Department of Biotechnology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, IR Iran
3Shahid Beheshti University. M.C. International Branch, Tehran, IR Iran
4Department of Microbiology, Imam Reza Hospital, Tabriz, IR Iran
Abstract
Background: The importance of this research was to determine the prevalence of efflux pump genes among Acinetobacter baumannii isolates from hospitalized patients in Imam Reza hospital in Tabriz, Iran.
Materials and Methods: This descriptive study was conducted in the Imam Reza hospital, Tabriz, IR Iran during June 2013 to March 2014. Twenty-six strains were isolated from female patients (42.6%) and thirty-five from male patients (57.4%). Clinical specimens were cultured for isolation of the microbial agents of A. baumannii. The isolated bacteria were identified using biochemical tests. Disk diffusion susceptibility test was used to determine the antimicrobial susceptibility, and E-test methods were also used. The prevalence of efflux pump genes was detected by PCR and sequencing methods.
Results: The resistance of A. baumannii isolates against tested antibiotics was analyzed as follows: 51 (84%) to trimethoprim-sulfamethoxazole, 59 (98%) to ceftazidime, 60 (99%) to ciprofloxacin, 29 (48%) to amikacin, 46 (77%) to gentamicin, 30 (50%) to tobramicin, , 60 (99%) to imipenem,, 60 (99%) to meropenem,, 60 (99%) to ceftriaxon,, 60 (99%) to cefepime,, 60 (99%) to ofloxacin, 6 (11%) to colistin. By using E-test, 45 (73.3%) to imipenem, 57 (93.3%) to ciprofloxacin, 23 (38%) to amikacin were also analyzed. The prevalence of adeA, adeB, adeC, and abeMgenes was 54 (88.5%), 61 (100%), 57 (93.9%), and 60 (98.3%), respectively.
Conclusion: The result of this study showed high incidence of AdeABC efflux pump in MDR A. baumannii isolates and the growing number of nosocomial infections associated with XDR A. baumannii complex, leading to difficulties in antibiotic therapy.
Keywords
A. baumannii; Efflux pumps; PCR
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