Firouzi-Dalvand, Leila, Pooladi, Mehdi, Nowroozi, Jamileh, Akhvan-Sepahi, Abbas, Hooshiyar, Mansooreh (2016). Presence of exoU and exoS Genes in Pseudomonas aeruginosa Isolated from Urinary Tract Infections. , 2(2), 8-11.
Leila Firouzi-Dalvand; Mehdi Pooladi; Jamileh Nowroozi; Abbas Akhvan-Sepahi; Mansooreh Hooshiyar. "Presence of exoU and exoS Genes in Pseudomonas aeruginosa Isolated from Urinary Tract Infections". , 2, 2, 2016, 8-11.
Firouzi-Dalvand, Leila, Pooladi, Mehdi, Nowroozi, Jamileh, Akhvan-Sepahi, Abbas, Hooshiyar, Mansooreh (2016). 'Presence of exoU and exoS Genes in Pseudomonas aeruginosa Isolated from Urinary Tract Infections', , 2(2), pp. 8-11.
Firouzi-Dalvand, Leila, Pooladi, Mehdi, Nowroozi, Jamileh, Akhvan-Sepahi, Abbas, Hooshiyar, Mansooreh Presence of exoU and exoS Genes in Pseudomonas aeruginosa Isolated from Urinary Tract Infections. , 2016; 2(2): 8-11.

Presence of exoU and exoS Genes in Pseudomonas aeruginosa Isolated from Urinary Tract Infections

Infection Epidemiology and Microbiology
Article 3, Volume 2, Issue 2 - Serial Number 3, 2016, Pages 8-11    PDF (1.07 M)
Authors
Leila Firouzi-Dalvand1; Mehdi Pooladi2; Jamileh Nowroozi1; Abbas Akhvan-Sepahi1; Mansooreh Hooshiyar* 3
1Department of Microbiology North Tehran Branch, Islamic Azad University, Tehran, IR Iran
2Department of Biology, School of Basic Sciences, Science and Research Branch, Islamic Azad University, Tehran, IR Iran
3Department of Chemistry, Applied and Scientific University, Pasargad, Iran
Abstract
Background: Pseudomonas aeruginosa is considered an opportunistic pathogen; several reports indicate that the organism can also cause infections in healthy hosts. Four effector proteins have been described in P. aeruginosa: exoU, exoS, exoT, and exoY. These genes that are translated into protein products related to type III secretion systems. Materials and Methods: A total of 134 samples were isolated, and P. aeruginosa was identified using biochemical tests. Bacterial genomic DNA was extracted, and the presence of the exoSand exoUgenes were detected by PCR. Biofilms were formed by culturing P. aeruginosaon glass slides in rich medium. Results: The exoU(73%), exoS (62%) genes were detected from infections caused by P. aeruginosa in urinary tract infection patients. Among the 119 strains isolated from patients with urinary tract infections. Conclusion: An improved understanding of virulence genes and biofilm formation in P.aeruginosa may facilitate the future development of novel vaccines and drug treatments.
Keywords
Pseudomonas aeruginosa; exoU; exoS; Urinary tract infection; Biofilm
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