Li, Q., Chao, T., Xuan, R., Chu, Y., Wang, Sh., Cheng, M., Li, P., Ji, Zh., Wang, J. (2024). Analysis of Matrix Metalloproteinase-9 Promoter Region Activity and Association Analysis of Promoter Region SNPs with Lactation Traits in Dairy Goats. , 26(4), 759-770. doi: 10.22034/JAST.26.4.759
Q. Li; T. Chao; R. Xuan; Y. Chu; Sh. Wang; M. Cheng; P. Li; Zh. Ji; J. Wang. "Analysis of Matrix Metalloproteinase-9 Promoter Region Activity and Association Analysis of Promoter Region SNPs with Lactation Traits in Dairy Goats". , 26, 4, 2024, 759-770. doi: 10.22034/JAST.26.4.759
Li, Q., Chao, T., Xuan, R., Chu, Y., Wang, Sh., Cheng, M., Li, P., Ji, Zh., Wang, J. (2024). 'Analysis of Matrix Metalloproteinase-9 Promoter Region Activity and Association Analysis of Promoter Region SNPs with Lactation Traits in Dairy Goats', , 26(4), pp. 759-770. doi: 10.22034/JAST.26.4.759
Li, Q., Chao, T., Xuan, R., Chu, Y., Wang, Sh., Cheng, M., Li, P., Ji, Zh., Wang, J. Analysis of Matrix Metalloproteinase-9 Promoter Region Activity and Association Analysis of Promoter Region SNPs with Lactation Traits in Dairy Goats. , 2024; 26(4): 759-770. doi: 10.22034/JAST.26.4.759

Analysis of Matrix Metalloproteinase-9 Promoter Region Activity and Association Analysis of Promoter Region SNPs with Lactation Traits in Dairy Goats

Journal of Agricultural Science and Technology
Article 4, Volume 26, Issue 4, 2024, Pages 759-770    PDF (6.54 M)
Document Type: Original Research
DOI: 10.22034/JAST.26.4.759
Authors
Q. Li1; T. Chao1; R. Xuan1; Y. Chu1; Sh. Wang1; M. Cheng2; P. Li2; Zh. Ji1; J. Wang* 1
1Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Tai’an City, Shandong Province, China.
2Qingdao Animal Husbandry and Veterinary Research Institution, Qingdao 266100, Shandong Province, China.
Abstract
Matrix Metalloproteinase-9 (MMP9) degrades the Extracellular Matrix (ECM), participates in mammary gland remodeling, and inhibits mammary epithelial cell apoptosis in goats. To investigate the transcriptional regulatory mechanism of the MMP9 promoter region, we analyzed the expression pattern of MMP9 in dairy goats by qRT‒PCR and cloned the promoter region by PCR. Deletion analysis indicated that the MMP9 gene core promoter region was located upstream of the transcription start site in the -715 bp to -926 bp region. We predicted three Specificity protein 1 (Sp1) binding sites in the MMP9 core promoter region, and performed targeted mutations on these three sites. The c.1863 G> A mutation in the MMP9 gene increased the promoter transcriptional activity and may be associated with an additional Serum Response Factor (SRF) transcription factor-binding site. Association analysis revealed that c.1863 G> A was significantly associated with milk fat percentage in dairy goats, which was significantly higher in goats with the AG genotype (4.71±0.02%) than in goats with the GG genotype (4.61±0.05%). This study lays a foundation for subsequent analysis of the transcriptional regulatory mechanism of MMP9 and exploration of its biological functions.
Keywords
Laoshan dairy goat; MMP9; SNP; Sp1
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