Boustanshenas, Mina, Akbari, Majid, Rezaie, Niloofar (2016). Evaluation of two different laboratory methods for the identification of Aeromonas spp. in stool sample of patients with diarrhea. , 2(1), 18-21.
Mina Boustanshenas; Majid Akbari; Niloofar Rezaie. "Evaluation of two different laboratory methods for the identification of Aeromonas spp. in stool sample of patients with diarrhea". , 2, 1, 2016, 18-21.
Boustanshenas, Mina, Akbari, Majid, Rezaie, Niloofar (2016). 'Evaluation of two different laboratory methods for the identification of Aeromonas spp. in stool sample of patients with diarrhea', , 2(1), pp. 18-21.
Boustanshenas, Mina, Akbari, Majid, Rezaie, Niloofar Evaluation of two different laboratory methods for the identification of Aeromonas spp. in stool sample of patients with diarrhea. , 2016; 2(1): 18-21.

Evaluation of two different laboratory methods for the identification of Aeromonas spp. in stool sample of patients with diarrhea

Infection Epidemiology and Microbiology
Article 6, Volume 2, Issue 1 - Serial Number 2, 2016, Pages 18-21    PDF (649 K)
Authors
Mina Boustanshenas1; Majid Akbari* 2; Niloofar Rezaie3
1Antimicrobial Resistance Research Center, Rasul-e-Akram Hospital, Iran University of Medical Sciences, Tehran, Iran
2Department of Microbiology, School of Nursing and midwifery, Arak University of Medical Sciences, Arak, Iran
3Department of Bacteriology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
Abstract
Background: Aeromonas spp. can cause diarrhea and various infections in humans. Access to rapid techniques with a high sensitivity and specificity is strongly needed for the identification of Aeromonas species. The aim of this study was to evaluate two different methods including API 20E bacterial identification tests and the molecular detection using PCR primers specific for 16s-rRNA and 23S-rRNA genes sequences for identification of Aeromonas spp. in stool samples from patients with diarrhea. Materials and Methods: One hundred stool samples from diarrheal patients were collected. All isolates were subjected toAPI 20 E strip tests and PCR using specific primers for identification of Aeromonas spp. Results: The API 20E analysis identified 2 (2.2%) isolates as Aeromonas spp. Molecular identification by aero-23S-rRNA gene confirmed the same 2 isolates as identified by the API 20E strips. Conclusion: Both API 20E system and PCR method using Aero 23S-rRNA primer were found to be accurate in identification of Aeromonas spp. isolates with highconfidence.
Keywords
API bacterial identification; Aeromonas spp; PCR
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