Saberi, F., Marzban, R., Ardjmand, M., Pajoum Shariati, F., Tavakoli, O. (2023). Optimization of the Culture Medium, Fermentation Process, and Effectiveness of a Biopesticide from an Iranian Bacillus thuringiensis var. tenebrionis (BN2). , 25(2), 469-484. doi: 10.52547/jast.25.2.469
F. Saberi; R. Marzban; M. Ardjmand; F. Pajoum Shariati; O. Tavakoli. "Optimization of the Culture Medium, Fermentation Process, and Effectiveness of a Biopesticide from an Iranian Bacillus thuringiensis var. tenebrionis (BN2)". , 25, 2, 2023, 469-484. doi: 10.52547/jast.25.2.469
Saberi, F., Marzban, R., Ardjmand, M., Pajoum Shariati, F., Tavakoli, O. (2023). 'Optimization of the Culture Medium, Fermentation Process, and Effectiveness of a Biopesticide from an Iranian Bacillus thuringiensis var. tenebrionis (BN2)', , 25(2), pp. 469-484. doi: 10.52547/jast.25.2.469
Saberi, F., Marzban, R., Ardjmand, M., Pajoum Shariati, F., Tavakoli, O. Optimization of the Culture Medium, Fermentation Process, and Effectiveness of a Biopesticide from an Iranian Bacillus thuringiensis var. tenebrionis (BN2). , 2023; 25(2): 469-484. doi: 10.52547/jast.25.2.469

Optimization of the Culture Medium, Fermentation Process, and Effectiveness of a Biopesticide from an Iranian Bacillus thuringiensis var. tenebrionis (BN2)

Journal of Agricultural Science and Technology
Article 17, Volume 25, Issue 2, 2023, Pages 469-484    PDF (1.26 M)
Document Type: Original Research
DOI: 10.52547/jast.25.2.469
Authors
F. Saberi1; R. Marzban* 2; M. Ardjmand3; F. Pajoum Shariati1; O. Tavakoli4
1Department of Chemical Engineering, Science and Research Branch, Islamic Azad University, Tehran, Islamic Republic of Iran.
2Iranian Research Institute of Plant Protection, Agricultural Research, Education and Extension Organization (AREEO), Tehran, Islamic Republic of Iran.
3Department of Chemical Engineering, Tehran South Branch, Islamic Azad University, Tehran, Islamic Republic of Iran.
4School of Chemical Engineering, College of Engineering, University of Tehran, Tehran, Islamic Republic of Iran.
Abstract
The local strain of B. thuringiensis var. tenebrionis-BN2 (Btt-BN2) was used to control the alfalfa weevil. Experimental design using Response Surface Methodology (RSM) was applied for the optimization of the culture medium and fermentation parameters in order to achieve a high level of Colony-Forming Unit (CFU) (spore mL−1). The parameters including the amount of carbon and nitrogen sources at three levels were investigated with CFU (spore mL−1) response for two sets of experiments. The obtained results showed that the maximum CFU (spore mL−1) for the minimum concentration of oat (2 g L-1), the minimum concentration of corn steep liquor (10 g L-1), and the maximum concentration of sugarcane molasses (10 g L-1) were equivalent to 1.4×1013 spore mL-1. Optimum fermentation parameters to obtain the highest value of CFU (spore mL−1) were determined as a maximum level of pH of eight and a medium level of temperature (280C). Amazingly, optimum conditions enhanced the CFU value to 8.06× 1013 spore mL-1, which is very significant in the Btt research. Finally, the bioassay analysis of Btt in a single system and binary system (combination of two insecticides; Matrine® and Abamectin®) at different concentrations illustrated 83% of mortality efficiency (3 ppm of Btt and 0.5 ppm Matrine) on the 3rd day of treatment and 100% efficiency almost for all combination of Btt with Matrine and/or Abamectin after the 7th day. The bioassay results showed promising environmentally friendly mortality efficiency compared to the current chemical treatments.
Keywords
Alfalfa weevil; Biological control; Fermentation parameters; Integrated pest management; mortality
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