Rapid detection of bacterial seed-borne disease Xanthomonas translucene in wheat using loop-mediated isothermal amplification (LAMP) assay in Iran | ||
| Journal of Crop Protection | ||
| Article 6, Volume 13, Issue 2, 2024, Pages 179-189 PDF (658.59 K) | ||
| Document Type: Original Research | ||
| DOI: 10.48311/jcp.2024.1664 | ||
| Authors | ||
| Fahimeh Nazari* ; Hossein Jafari; Abolghasem Ghasemi | ||
| Department of Plant Disease Research, Iranian Research Institute of Plant Protection (IRIPP), Agricultural Research Education and Extension Organization (AREEO), Tehran, Iran. | ||
| Abstract | ||
| Bacterial leaf streak (BLS) is one of the most important seed borne diseases of cereals, especially in wheat and barley. This disease can cause yield loss of up to 40% by producing dark brown spots on the glumes, called black chaff. Although this disease has spread widely in Iran recently, a quick and economical method for molecular and accurate detection of the disease agent, Xanthomonas translucene, has not been identified in the country. Quick and accurate detection of the bacteria in seed masses plays an important role in disease management. In this research, the development of a diagnostic kit for the causal agent of wheat bacterial leaf streak disease based on LAMP (Loop Mediated Isothermal Amplification) was performed by designing specific primers based on two new housekeeping genes rpoD and dnaK, which have not been used as LAMP targets previously). The serial dilution method of the bacterium was used to determine the sensitivity of the designed primers. The efficiency of the diagnostic kit was evaluated by collecting commercial wheat seed masses from different regions of the country and using the pure culture of the bacteria. The results showed that the primers designed based on the rpoD gene were the most effective. These primers detected up to 3.5 x 102 bacteria cells in one milliliter. The specificity of the primers was proved by performing the LAMP reaction on different genera of wheat pathogenic bacteria. The results of this project can be used for the rapid detection of X. translucense in wheat seeds and can be used as one of the management strategies for preparing healthy seeds. | ||
| Keywords | ||
| Bacterial leaf streak disease; kit; LAMP | ||
| References | ||
|
| ||
|
Statistics Article View: 93 PDF Download: 71 |
||
| Number of Journals | 45 |
| Number of Issues | 2,171 |
| Number of Articles | 24,674 |
| Article View | 24,431,295 |
| PDF Download | 17,549,886 |