Bayram, M. E., Korkut, K. Z. (2019). Determination of Allelic Compositions of Glu-1 and Glu-3 Loci in Bread Wheat Genotypes. , 21(4), 981-992.
M. E. Bayram; K. Z. Korkut. "Determination of Allelic Compositions of Glu-1 and Glu-3 Loci in Bread Wheat Genotypes". , 21, 4, 2019, 981-992.
Bayram, M. E., Korkut, K. Z. (2019). 'Determination of Allelic Compositions of Glu-1 and Glu-3 Loci in Bread Wheat Genotypes', , 21(4), pp. 981-992.
Bayram, M. E., Korkut, K. Z. Determination of Allelic Compositions of Glu-1 and Glu-3 Loci in Bread Wheat Genotypes. , 2019; 21(4): 981-992.

Determination of Allelic Compositions of Glu-1 and Glu-3 Loci in Bread Wheat Genotypes

Journal of Agricultural Science and Technology
Article 16, Volume 21, Issue 4, 2019, Pages 981-992    PDF (385.83 K)
Document Type: Original Research
Authors
M. E. Bayram* 1; K. Z. Korkut2
1Maize Research Institute, 54580 Sakarya, Turkey.
2Department of Field Crops, Faculty of Agriculture, Namık Kemal University, 59030 Tekirdağ, Turkey.
Abstract
Breeding high-quality wheat cultivars is one of the main targets of breeding programs. Identification of High Molecular Weight Glutenin Subunits (HMW-GS) and Low Molecular Weight Glutenin Subunits (LMW-GS) alleles that confer high quality may be one of the easy ways to determine promising wheat lines in early generations. HMW-GS and LMW-GS alleles of 64 bread wheat (Triticum aestivum L.) genotypes from a Turkish breeding program including advanced lines and cultivars were identified using Sodium-Dodecyl-Sulfate PolyAcrylamide Gel (SDS-PAGE) electrophoresis method. In total, 26 alleles were identified including three alleles at the Glu-A1 locus, 6 alleles at the Glu-B1 locus, 2 alleles at the Glu-D locus, 6 alleles at the Glu-A3 locus, 7 at the Glu-B3 locus, and 2 alleles at the Glu-D3 locus. The most prevalent alleles among the 64 genotypes in the study were 2* (67.2%), 7+9 (42.2%), 5+10 (68.8%), A3c (28.1%), B3b (35.9%), and D3c (92.2%). The previously announced good flour quality conferring alleles 1, 17+18, 5+10 at Glu-1 loci and A3b, A3c, B3b, B3c, B3d, B3g at Glu-3 loci were also identified in the present study, indicating that these alleles can be used as markers for selection of higher-quality genotypes in wheat breeding programs. Introduction of the allele structure of the studied genotypes may enlighten the way for the wheat breeders in the crossing decisions.
Keywords
Flour quality; Glutenin subunits; Plant breeding; SDS-PAGE
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