Karami, Z., Peighambardoust, S. H., Hesari, J., Akbari-Adergani, B. (2019). Response Surface Methodology to Optimize Hydrolysis Parameters in Production of Antioxidant Peptides from Wheat Germ Protein by Alcalase Digestion and Identification of Antioxidant Peptides by LC-MS/MS. , 21(4), 829-844.
Z. Karami; S. H. Peighambardoust; J. Hesari; B. Akbari-Adergani. "Response Surface Methodology to Optimize Hydrolysis Parameters in Production of Antioxidant Peptides from Wheat Germ Protein by Alcalase Digestion and Identification of Antioxidant Peptides by LC-MS/MS". , 21, 4, 2019, 829-844.
Karami, Z., Peighambardoust, S. H., Hesari, J., Akbari-Adergani, B. (2019). 'Response Surface Methodology to Optimize Hydrolysis Parameters in Production of Antioxidant Peptides from Wheat Germ Protein by Alcalase Digestion and Identification of Antioxidant Peptides by LC-MS/MS', , 21(4), pp. 829-844.
Karami, Z., Peighambardoust, S. H., Hesari, J., Akbari-Adergani, B. Response Surface Methodology to Optimize Hydrolysis Parameters in Production of Antioxidant Peptides from Wheat Germ Protein by Alcalase Digestion and Identification of Antioxidant Peptides by LC-MS/MS. , 2019; 21(4): 829-844.

Response Surface Methodology to Optimize Hydrolysis Parameters in Production of Antioxidant Peptides from Wheat Germ Protein by Alcalase Digestion and Identification of Antioxidant Peptides by LC-MS/MS

Journal of Agricultural Science and Technology
Article 5, Volume 21, Issue 4, 2019, Pages 829-844    PDF (1.9 M)
Document Type: Original Research
Authors
Z. Karami1; S. H. Peighambardoust* 1; J. Hesari1; B. Akbari-Adergani2
1Department of Food Science, College of Agriculture, University of Tabriz, Tabriz 5166616471, Islamic Republic of Iran.
2Food and Drug Laboratory Research Center, Food and Drug Organization, Ministry of Health and Medical Education, Tehran 11136-15911, Islamic Republic of Iran.
Abstract
The antioxidant capacity of wheat germ protein hydrolyzed by Alcalase was optimized using Response Surface Methodology (RSM). The optimum hydrolyzing parameters were found at temperature of 52.28°C, time 233 minutes, and E/S 1.46 %. The amino acids profiles of intact and hydrolyzed proteins showed that Wheat Germ Protein Hydrolysate (WGPH) had higher percentage of hydrophobic amino acids than that of intact protein. WGPH prepared in optimum condition was fractionated by RP-HPLC. The obtained fractions were subjected to ABTS assay for antioxidant capacity evaluation. The fraction with higher antioxidant value was then exposed to further analysis by LC-ESI/MS/MS. The sequences of the peptides were found to be TVGGAPAGRIVME (1257.66 Da) and GNPIPREPGQVPAY (1494.77 Da).
Keywords
ABTS assay; Amino acids profiles; Bioactive peptides; RSM
References
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