Azizi, M., Kaboli Farshchi, H., Oroojalian, F., Orafaee, H. (2017). Green Synthesis of Silver Nano-particles Using Kelussia odoratissima Mozaff. Extract and Evaluation of its Antibacterial Activity. , 19(3), 681-691.
M. Azizi; H. Kaboli Farshchi; F. Oroojalian; H. Orafaee. "Green Synthesis of Silver Nano-particles Using Kelussia odoratissima Mozaff. Extract and Evaluation of its Antibacterial Activity". , 19, 3, 2017, 681-691.
Azizi, M., Kaboli Farshchi, H., Oroojalian, F., Orafaee, H. (2017). 'Green Synthesis of Silver Nano-particles Using Kelussia odoratissima Mozaff. Extract and Evaluation of its Antibacterial Activity', , 19(3), pp. 681-691.
Azizi, M., Kaboli Farshchi, H., Oroojalian, F., Orafaee, H. Green Synthesis of Silver Nano-particles Using Kelussia odoratissima Mozaff. Extract and Evaluation of its Antibacterial Activity. , 2017; 19(3): 681-691.

Green Synthesis of Silver Nano-particles Using Kelussia odoratissima Mozaff. Extract and Evaluation of its Antibacterial Activity

Journal of Agricultural Science and Technology
Article 15, Volume 19, Issue 3, 2017, Pages 681-691    PDF (638.46 K)
Authors
M. Azizi* 1; H. Kaboli Farshchi1; F. Oroojalian2; H. Orafaee3
1Department of Horticulture, College of Agriculture, Ferdowsi University of Mashhad, Mashhad, Islamic Republic of Iran.
2Department of Life Science Engineering, Faculty of New Sciences and Technologies, University of Tehran, Tehran, Islamic Republic of Iran.
3Pharmaceutics Department, Faculty of Pharmacy, Mashhad University of Medical Science, Mashhad, Islamic Republic of Iran.
Abstract
In this research Kelussia odoratissima Mozaff. leaf extract was used for the green synthesis of silver nanoparticles (AgNPs). At first we compared antioxidant activity of different extracts of K. odoratissima. Then solution containing silver nitrate was treated with the extract which showed high antioxidant activity. Synthesized AgNPs were evaluated by analyzing the excitation of surface plasmon resonance. TEM analysis was also used for nanoparticle characterization. Antibacterial activity of the solution containing AgNPs was measured by microdilution test. Common food contaminant bacteria such as gram-positive (Staphylococcus aureus, Bacillus cereus, Listeria monocytogenes) and gram-negative (Escherichia coli O157: H7, Salmonella enterica and Pseudomonas aeruginosa)were used for the evaluation. The aqueous extract showed the highest antioxidant activity and the solution was used for the green synthesis of AgNPs. The particle diameters were calculated to be 20-40nm with -17 to -19.9 mV zeta potential. The TEM micrographs showed that the AgNPs are nearly spherical in shape and highly monodispersed. MIC of the AgNPs against gram-positive and gram-negative bacteria was between 0.012-0.025 and 0.006-0.012 mg/ml respectively.
Keywords
Kelussia odoratissima; Green synthesis; silver nanoparticles; Antibacterial activity; Electron microscopy
References
Statistics
Article View: 158
PDF Download: 68
Statistics
Number of Journals45
Number of Issues2,171
Number of Articles24,674
Article View24,437,034
PDF Download17,551,643