Zarred, K., Laarif, A., Ben Hamouda, A., Chaieb, I., Mediouni-Ben Jemaa, J. (2017). Anticholinesterase Potential of Monoterpenoids on the Whitefly Bemisia tabaci and Their Kinetic Studies. , 19(3), 643-652.
K. Zarred; A. Laarif; A. Ben Hamouda; I. Chaieb; J. Mediouni-Ben Jemaa. "Anticholinesterase Potential of Monoterpenoids on the Whitefly Bemisia tabaci and Their Kinetic Studies". , 19, 3, 2017, 643-652.
Zarred, K., Laarif, A., Ben Hamouda, A., Chaieb, I., Mediouni-Ben Jemaa, J. (2017). 'Anticholinesterase Potential of Monoterpenoids on the Whitefly Bemisia tabaci and Their Kinetic Studies', , 19(3), pp. 643-652.
Zarred, K., Laarif, A., Ben Hamouda, A., Chaieb, I., Mediouni-Ben Jemaa, J. Anticholinesterase Potential of Monoterpenoids on the Whitefly Bemisia tabaci and Their Kinetic Studies. , 2017; 19(3): 643-652.

Anticholinesterase Potential of Monoterpenoids on the Whitefly Bemisia tabaci and Their Kinetic Studies

Journal of Agricultural Science and Technology
Article 12, Volume 19, Issue 3, 2017, Pages 643-652    PDF (612.64 K)
Authors
K. Zarred* 1; A. Laarif2; A. Ben Hamouda2; I. Chaieb3; J. Mediouni-Ben Jemaa4
1Institut Supérieur Agronomique of Chott Mariem (ISA), University of Sousse, Tunisia.
2UR13AGR09, Regional Center for Research in Horticulture and Biological Agriculture (CRRHAB), University of Sousse, Tunisia.
3Laboratory of Plant Protection, National Institute of Agronomic Research of Tunisia (INRAT), University of Carthage, Tunisia.
4Laboratory of Biotechnology Applied to Agriculture, National Institute of Agronomic Research of Tunisia (INRAT), Tunisia.
Abstract
B-biotype Bemisia tabaci is a severe insect pest worldwide in many ornamental, agricultural, and horticultural crops. Control of this insect is obstructed by resistance to many AcetylCholinEsterase (AChE)-inhibiting insecticides, such as organophosphates and carbamates. In the present work, we evaluated the acetylcholinesterase inhibitory activity of six monoterpenoids namely α-pinene, terpineol, linalool, ß-myrcene, nerol and geraniol in vitro and in vivo. Inhibition of AChE of B. tabaci was measured by colorimetric method. The results showed that all of the monoterpenoids produced AChE inhibitory activity, with IC50 values ranging from 0.96 to 26.85 mM. Alpha-pinene showed the most potent inhibitory activity (IC50= 0.96 mM). Kinetic analysis showed reversible non-competitive type inhibition, revealing that these components might bind both the enzyme alone and the enzyme-substrate. Results demonstrate the AChE inhibitory activity as mode of action of these monoterpenoids at relatively high concentrations. Thus, this could be useful for investigation of new ecofriendly natural insecticidal compounds.
Keywords
Acetylcholinesterase; Bemisia tabaci; IPM; Monoterpenoids; Non-competitive inhibition
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