Ahmed, I., Hayat, R., Iqbal, M., Bibi, N., Khalid, N. (2016). Molecular Identification and Characterization of Phosphate Solubilizing Pseudomonas sp. Isolated from Rhizosphere of Mash Bean (Vigna Mungo L.) for Growth Promotion in Wheat. , 18(3), 775-788.
I. Ahmed; R. Hayat; M. Iqbal; N. Bibi; N. Khalid. "Molecular Identification and Characterization of Phosphate Solubilizing Pseudomonas sp. Isolated from Rhizosphere of Mash Bean (Vigna Mungo L.) for Growth Promotion in Wheat". , 18, 3, 2016, 775-788.
Ahmed, I., Hayat, R., Iqbal, M., Bibi, N., Khalid, N. (2016). 'Molecular Identification and Characterization of Phosphate Solubilizing Pseudomonas sp. Isolated from Rhizosphere of Mash Bean (Vigna Mungo L.) for Growth Promotion in Wheat', , 18(3), pp. 775-788.
Ahmed, I., Hayat, R., Iqbal, M., Bibi, N., Khalid, N. Molecular Identification and Characterization of Phosphate Solubilizing Pseudomonas sp. Isolated from Rhizosphere of Mash Bean (Vigna Mungo L.) for Growth Promotion in Wheat. , 2016; 18(3): 775-788.

Molecular Identification and Characterization of Phosphate Solubilizing Pseudomonas sp. Isolated from Rhizosphere of Mash Bean (Vigna Mungo L.) for Growth Promotion in Wheat

Journal of Agricultural Science and Technology
Article 16, Volume 18, Issue 3, 2016, Pages 775-788    PDF (1.06 M)
Authors
I. Ahmed* 1; R. Hayat2; M. Iqbal1; N. Bibi1, 2; N. Khalid3
1Institute of Microbial Culture Collection of Pakistan (IMCCP), National Agricultural Research Center, Islamabad-45500, Pakistan.
2Department of Soil Science and SWC, Pir Mehr Ali Shah Arid Agriculture University, Rawalpindi, Pakistan.
3Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.
Abstract
Bio-inoculants have potential role in plant growth promotion. The present study evaluated the potential of Pseudomonas strains as bio-inoculants in wheat on the basis of plant growth promotion and physiological characterization. The 16S rRNA gene sequencing and phylogenetic analysis revealed that four isolated strains belonged to genus Pseudomonas. These strains were positive for phosphorus solubilization and indole acetic acid production, whereas only two strains were positive candidate for their nitrogen fixing ability as determined by presence or absence of nifH gene through amplification from polymerase chain reaction. The pot experiment showed that the integrated use of Pseudomonas strains as co-inoculant and 50% applied mineral fertilizers enhanced the maximum wheat growth and development from 58 to 140% for different shoot and root growth parameters. The strain NCCP-45 and NCCP-237 were closely related to Pseudomonas beteli and Pseudomonas lini, respectively. These isolated strains can be used to increase crop productivity by using as a bio-fertilizer inoculum.
Keywords
Bio-inoculant; PCR; PGPR; Phylogenetic analysis; 16S rRNA gene
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