Liu, X., Wu, J. H., Gao, Y., Wu, Y., Qian, L., Wang, Zh. (2015). Characterization of Structure and Cellular Immunity Bioactivity of Milk-Derived Galactooligosacchrides Prepared by Lactobacillus delbrueckii subsp. bulgaricus Fermentation. , 17(6), 1481-1494.
X. Liu; J. H. Wu; Y. Gao; Y. Wu; L. Qian; Zh. Wang. "Characterization of Structure and Cellular Immunity Bioactivity of Milk-Derived Galactooligosacchrides Prepared by Lactobacillus delbrueckii subsp. bulgaricus Fermentation". , 17, 6, 2015, 1481-1494.
Liu, X., Wu, J. H., Gao, Y., Wu, Y., Qian, L., Wang, Zh. (2015). 'Characterization of Structure and Cellular Immunity Bioactivity of Milk-Derived Galactooligosacchrides Prepared by Lactobacillus delbrueckii subsp. bulgaricus Fermentation', , 17(6), pp. 1481-1494.
Liu, X., Wu, J. H., Gao, Y., Wu, Y., Qian, L., Wang, Zh. Characterization of Structure and Cellular Immunity Bioactivity of Milk-Derived Galactooligosacchrides Prepared by Lactobacillus delbrueckii subsp. bulgaricus Fermentation. , 2015; 17(6): 1481-1494.

Characterization of Structure and Cellular Immunity Bioactivity of Milk-Derived Galactooligosacchrides Prepared by Lactobacillus delbrueckii subsp. bulgaricus Fermentation

Journal of Agricultural Science and Technology
Article 9, Volume 17, Issue 6, 2015, Pages 1481-1494    PDF (713.83 K)
Authors
X. Liu; J. H. Wu* ; Y. Gao; Y. Wu; L. Qian; Zh. Wang
Department of Food Science and Technology, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, People's Republics of China.
Abstract
In this study, the milk-derived GalactoOligoSaccharides (GOS) were produced by Lactobacillus delbrueckii subsp. bulgaricus and refined by an ultrafiltration-nanofiltration continuous membrane. By further investigation, we found that the GOS product purified by gel permeation chromatography mainly contained low molecular weight disaccharide and trisaccharide, that is, 4-β-galactobiose and tri-galacto-oligosaccharides. The cellular immune activity of the purified GOS was evaluated by using Intestinal Epithelial Cells (IECs). Results showed that GOS could significantly (P< 0.05) promote IECs proliferation in a dose and time dependent manner, and the relative proliferation rate after 24 hours culture was high up to 158% at the concentration of 100 μg mL-1, which was three time the value after 4 hours culture without GOS. Moreover, the production of IL-6 was observably increased and up to 133.54 ng L-1 with addition of 100 μg mL-1 GOS. These data implied that the purified GOS might have a role in promoting the immune adjustment, which could be utilized as a novel and natural immunoregulatory agent in the field of medicine and functional food. This work also revealed that the employment of transgalactosylation activity of β-galactosidase derived from the fermentation of probiotics such as Lactobacillus delbrueckii subsp. bulgaricus would enhance the value of the milk product due to the form of GOS.
Keywords
Fermentation; Galacto-oligasacchrides; Immunoregulatory activity; probiotics
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