Mojazi Amiri, B., Maebayashi, M., Omoto, N., Adachi, S., Yamauchi, K. (2001). In Vitro Oocyte Maturation in a Hybrid Sturgeon, Bester: Changes in the Germinal Vesicle Breakdown and 17, 20β- dihydroxy-4-pregnen-3-one Production. , 3(3), 199-207.
B. Mojazi Amiri; M. Maebayashi; N. Omoto; S. Adachi; K. Yamauchi. "In Vitro Oocyte Maturation in a Hybrid Sturgeon, Bester: Changes in the Germinal Vesicle Breakdown and 17, 20β- dihydroxy-4-pregnen-3-one Production". , 3, 3, 2001, 199-207.
Mojazi Amiri, B., Maebayashi, M., Omoto, N., Adachi, S., Yamauchi, K. (2001). 'In Vitro Oocyte Maturation in a Hybrid Sturgeon, Bester: Changes in the Germinal Vesicle Breakdown and 17, 20β- dihydroxy-4-pregnen-3-one Production', , 3(3), pp. 199-207.
Mojazi Amiri, B., Maebayashi, M., Omoto, N., Adachi, S., Yamauchi, K. In Vitro Oocyte Maturation in a Hybrid Sturgeon, Bester: Changes in the Germinal Vesicle Breakdown and 17, 20β- dihydroxy-4-pregnen-3-one Production. , 2001; 3(3): 199-207.

In Vitro Oocyte Maturation in a Hybrid Sturgeon, Bester: Changes in the Germinal Vesicle Breakdown and 17, 20β- dihydroxy-4-pregnen-3-one Production

Journal of Agricultural Science and Technology
Article 5, Volume 3, Issue 3, 2001, Pages 199-207    PDF (210.34 K)
Authors
B. Mojazi Amiri* 1; M. Maebayashi2; N. Omoto2; S. Adachi3; K. Yamauchi3
1Department of Fisheries and Environmental Sciences, Faculty of Natural Resources, University of Tehran, Karaj, 31585-4314, Islamic Republic of Iran,
2Department of Research and Development, The Hokkaido Electric Power Co., Inc., Sapporo 004, Japan.
3Department of Biology, Faculty of Fisheries, Hokkaido University, Hakodate 041, Japan.
Abstract
In order to determine the most effective hormones on the in vitro oocyte maturation
(germinal vesicle breakdown - GVBD) in a hybrid sturgeon bester, ovarian follicles were
incubated at the migratory nucleus stage in the presence of several steroids, gonadotropin
preparations and forskolin. Occurrence of GVBD and 17,20β-dihydroxy-4-pregnen-3 -one
(DHP) production were followed during an experimental period of one year of germinal
vesicle (GV) migration. From all the steroids tested, three progesterone derivatives-17α-
hydroxyprogesterone (17αOHP), 17, 20β, 21-trihydroxy-4-pregnen-3-one (17, 20 β, 21-
triol), and DHP-were the most potent steroid inducers of GVBD, followed closely by deoxycorticosterone
(DOC). No GVBD responses were found in ovarian follicles in July
when the GV was still central. The responsiveness increased gradually from then and
reached its peak in November when the GV had migrated fully towards the oocyte periphery.
Their potencys gradually declined later from December and the oocytes lost their
ability to mature the next July when degeneration set in. DHP production by ovarian follicle
during successive months of nucleus migration demonstrated a relatively similar pattern
of GVBD frequencies. These results indicated again a relevant role for DHP on oocyte
maturation in the bester and also suggested that GV localization along with in vitro
oocyte maturation assay can be used as practical tools for selecting the appropriate individuals
in exogenous induced spawning in bester.
Keywords
In vitro; Bester; DHP; GVBD; Maturation
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