Moradi, Zohreh, Mehrvar, Mohsen (2021). Incidence and molecular characterization of Beet virus Q in sugar beet production areas of Iran based on coat protein gene. , 10(3), 557-564. doi: 10.48311/jcp.2021.1556
Zohreh Moradi; Mohsen Mehrvar. "Incidence and molecular characterization of Beet virus Q in sugar beet production areas of Iran based on coat protein gene". , 10, 3, 2021, 557-564. doi: 10.48311/jcp.2021.1556
Moradi, Zohreh, Mehrvar, Mohsen (2021). 'Incidence and molecular characterization of Beet virus Q in sugar beet production areas of Iran based on coat protein gene', , 10(3), pp. 557-564. doi: 10.48311/jcp.2021.1556
Moradi, Zohreh, Mehrvar, Mohsen Incidence and molecular characterization of Beet virus Q in sugar beet production areas of Iran based on coat protein gene. , 2021; 10(3): 557-564. doi: 10.48311/jcp.2021.1556

Incidence and molecular characterization of Beet virus Q in sugar beet production areas of Iran based on coat protein gene

Journal of Crop Protection
Article 12, Volume 10, Issue 3, 2021, Pages 557-564    PDF (258.77 K)
Document Type: Original Research
DOI: 10.48311/jcp.2021.1556
Authors
Zohreh Moradi* 1; Mohsen Mehrvar2
1Department of Plant Pathology, Faculty of Crop Sciences, Sari Agricultural Sciences and Natural Resources University, Sari, Iran.
2Department of Plant Pathology, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, Iran.
Abstract
Beet virus Q (BVQ) is a soilborne pomovirus (family Virgaviridae) associated with rhizomania syndrome in sugar beet. In the present study, BVQ was investigated in sugar beet farms of 12 provinces in Iran by RT-PCR. Thirty-five out of 214 root samples resulted in a positive reaction to BVQ (16.3%). Moreover, 501-bp- long fragments of the coat protein gene of 11 Iranian isolates were purified, cloned, and sequenced. Phylogenetic analysis using the 501 bp fragment of 17 BVQ isolates (11 from this study and six retrieved from GenBank) showed that all isolates clustered in two main groups. Iranian isolates belonged to group I alongside isolates from France (AJ810289) and Germany (AJ223597). Iranian isolates shared 98.80–100% nucleotide sequence identity (98.19–100% amino acid identity) and 97.21–99.60% nucleotide sequence identity (96.99–99.40% amino acid identity) with the corresponding sequence of six other BVQ isolates available in the GenBank. Iranian isolates displayed the highest nucleotide and amino acid sequence identities of 98.80-99.60% and 98.19-99.40%, respectively, with the French isolate FP71 (AJ810289). To our knowledge, this is the first molecular characterization of BVQ in Iran. This information can be used in plant breeding to obtain virus-resistant plants.
Keywords
Soilborne virus; Sugar beet; RT-PCR; Beet virus Q
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