Antigenic Analysis of the Coat Protein of Alfalfa mosaic virus and its Involvement in Aphid Transmission

	Antigenic Analysis of the Coat Protein of Alfalfa mosaic virus and its Involvement in Aphid Transmission
Journal of Agricultural Science and Technology
Article 4, Volume 7, Issue 1, 2005, Pages 31-40 PDF (214.95 K)
Abstract
Two strains -425 and Y87/47- of Alfalfa mosaic virus (AMV) were propagated in and purified from Nicotiana tabacum cv. Samsun NN. Thirty-three AMV specific monoclonal antibodies (MAbs) from two fusions were raised against strain 425. These antibodies were of isotypes IgG1 and IgM. MAbs recognised three types of epitope. Group I did not react with the virus particle surface or viral coat protein of two strains in PTA-ELISA, but they reacted with a 30-kDa structural coat protein of AMV by immunoblot analysis only and were able to recognise cryptotopes. Group II reacted with metatopes of both strains in PTA-ELISA. Group III reacted with a 30-kDa structural coat protein of AMV by immunoblot analysis and in PTA-ELISA for the Y87/47 strain only. Immunoblocking experiments in which suspensions of purified AMV and MAb were offered between parafilm membranes for acquisition by Myzus persicae revealed that MAb-2 was effective in blocking (inhibiting) transmission. This result suggests that the epitope which was localised by MAb-2 plays a role in the aphid transmission of AMV.
Keywords
ELISA; Immunoblot; Immunoblocking; monoclonal antibody; Transmission
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