Serological Characterization of Alfalfa Mosaic Virus in Alfalfa (Medicago sativa) in Some Regions of Iran | ||
| Journal of Agricultural Science and Technology | ||
| Article 8, Volume 9, Issue 4, 2007, Pages 341-347 PDF (143.89 K) | ||
| Authors | ||
| H. Massumi* ; A. Hosseini Pour | ||
| Department of Plant Pathology, Faculty of Agriculture, Shahid Bahonar University of Kerman, P. O. Box: 76169-133, Kerman, Islamic Republic of Iran. | ||
| Abstract | ||
| A survey was carried out in five provinces of Iran (Kerman, Sistan and Baluchestan, Hormozgan, Khorasan and Yazd) for the presence of Alfalfa mosaic virus (AMV) sero-types in alfalfa during 2002 to 2003. The number of samples collected was 250, represent-ing the diversity and geographical distribution of AMV in these areas. Diagnosis was car-ried out using polyclonal (PAbs) and monoclonal (MAbs) antibodies. A total of 110 symp-tomatic leaf samples gave a positive reaction in ELISA with polyclonal antibodies. Twelve out of 20 MAbs reacted with all samples tested and were considered as non-differentiating MAbs. Only the MAbs-12, 13, 15, 21, 22 and 24 gave a clear differential reaction and were used for identifying AMV serotypes. Two MAbs (1 and 2) did not react with AMV posi-tive samples. Serological relatedness among AMV samples was studied by indicating the existence of six serotypes of AMV strains in the surveyed areas. | ||
| Keywords | ||
| AMV; Monoclonal antibodies; Serotype; Strain | ||
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