Chitinolytic Activity of Native Pseudomonas fluorescens Strains | ||
| Journal of Agricultural Science and Technology | ||
| Article 7, Volume 9, Issue 1, 2007, Pages 61-68 PDF (233.95 K) | ||
| Authors | ||
| R. Nandakumar* 1; S. Babu1; T. Raguchander2; R. Samiyappan1 | ||
| 1Department of Plant Pathology, Centre for Plant Protection Studies, Tamil Nadu Agricultural University, Coimbatore 640 003, India. | ||
| 2Sugarcane Research Station, Semmandalam, Cuddalore 607 001, Tamil Nadu, India. | ||
| Abstract | ||
| The chitinase producing ability of Pseudomonas fluorescens strains viz., PF1, PB2 and FP7 was evaluated in a culture medium with and without a chitin source. The addition of 1% (v/v) chitin in culture medium significantly increased the bacterial population and chitinase activity. Among three strains tested, FP7 responded well to the addition of chitin by producing 31.2% increased chitinase in culture. Western blot analysis with chitinase antibody detected six and five chitinase isoforms in culture inoculated with FP7 and PF1, respectively. | ||
| Keywords | ||
| Biological control; Chitin; Chitinase; Pseudomonas fluorescens | ||
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