Mahmoudi Nasab, H., Mardi, M., Talaee, H., Fazeli Nashli, H., Pirseyedi, S. M., Hejabri Noubari, A., Mowla*, S. J. (2010). Molecular Analysis of Ancient DNA Extracted from 3250-3450 Year-old Plant Seeds Excavated from Tepe Sagz Abad in Iran. , 12(4), 459-470.
H. Mahmoudi Nasab; M. Mardi; H. Talaee; H. Fazeli Nashli; S. M. Pirseyedi; A. Hejabri Noubari; S. J. Mowla*. "Molecular Analysis of Ancient DNA Extracted from 3250-3450 Year-old Plant Seeds Excavated from Tepe Sagz Abad in Iran". , 12, 4, 2010, 459-470.
Mahmoudi Nasab, H., Mardi, M., Talaee, H., Fazeli Nashli, H., Pirseyedi, S. M., Hejabri Noubari, A., Mowla*, S. J. (2010). 'Molecular Analysis of Ancient DNA Extracted from 3250-3450 Year-old Plant Seeds Excavated from Tepe Sagz Abad in Iran', , 12(4), pp. 459-470.
Mahmoudi Nasab, H., Mardi, M., Talaee, H., Fazeli Nashli, H., Pirseyedi, S. M., Hejabri Noubari, A., Mowla*, S. J. Molecular Analysis of Ancient DNA Extracted from 3250-3450 Year-old Plant Seeds Excavated from Tepe Sagz Abad in Iran. , 2010; 12(4): 459-470.

Molecular Analysis of Ancient DNA Extracted from 3250-3450 Year-old Plant Seeds Excavated from Tepe Sagz Abad in Iran

Journal of Agricultural Science and Technology
Article 8, Volume 12, Issue 4, 2010, Pages 459-470    PDF (867.43 K)
Authors
H. Mahmoudi Nasab1; M. Mardi2; H. Talaee3; H. Fazeli Nashli3; S. M. Pirseyedi2; A. Hejabri Noubari* 4; S. J. Mowla*1
1Department of Genetics, Faculty of Basic Science, Tarbiat Modares University, P. O. Box: 14115-175, Tehran, Islamic Republic of Iran.
2Department of Genomics, Agricultural Biotechnology Research Institute, Karaj, Islamic Republic of Iran.
3Department of Archaeology, Faculty of Letters and Humanities, Tehran University, Tehran, Islamic Republic of Iran.
4Department of Archaeology, Faculty of Humanities, Tarbiat Modares University, Tehran, Islamic Republic of Iran.
Abstract
To determine ancient seed species, 3250-3450 year-old charred seeds obtained from different Iranian archaeological sites were studied using Scanning Electron Microscopy (SEM) and molecular analysis. SEM analysis of ancient seeds revealed that the surface of the seeds was damaged. Therefore, we could not accurately identify their species. Molecular analysis on ancient specimens was done on different samples obtained from Masjede Kabood (Tabriz), Tepe Rahmat Abad (Pasargad) and Tepe Sagz Abad (Qazvin plain). The specific primer pairs were designed based on a part of the promoter region of the High Molecular Weight (HMW) glutenin gene and a short fragment of the vrs1 gene were verified on samples of modern wheat and barley varieties, respectively. The designated primers failed to amplify ancient DNAs (aDNAs) obtained from Masjede Kabood and Tepe Rahmat Abad, but successfully amplified the aDNA obtained from Tepe Sagz Abad. This finding was expected since the latter seeds had a better morphological preservation in comparison to the former ones. The accuracy of the amplified products was further proved by cloning and sequencing.
Keywords
Wheat; Barley; Seed; aDNA; Ancient DNA; Molecular analyses
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