Hosseinzadeh Gharajeh, N., Hejazi, M. A., Nazeri, S., Barzegari, Abolfazl (2012). Characterization of an Indigenous Isolate, Dunaliella tertiolecta ABRIINW-G3, from Gavkhooni Salt Marsh in Iran Based on Molecular and Some Morpho-physiological Attributes. , 14(7), 1579-1590.
N. Hosseinzadeh Gharajeh; M. A. Hejazi; S. Nazeri; Abolfazl Barzegari. "Characterization of an Indigenous Isolate, Dunaliella tertiolecta ABRIINW-G3, from Gavkhooni Salt Marsh in Iran Based on Molecular and Some Morpho-physiological Attributes". , 14, 7, 2012, 1579-1590.
Hosseinzadeh Gharajeh, N., Hejazi, M. A., Nazeri, S., Barzegari, Abolfazl (2012). 'Characterization of an Indigenous Isolate, Dunaliella tertiolecta ABRIINW-G3, from Gavkhooni Salt Marsh in Iran Based on Molecular and Some Morpho-physiological Attributes', , 14(7), pp. 1579-1590.
Hosseinzadeh Gharajeh, N., Hejazi, M. A., Nazeri, S., Barzegari, Abolfazl Characterization of an Indigenous Isolate, Dunaliella tertiolecta ABRIINW-G3, from Gavkhooni Salt Marsh in Iran Based on Molecular and Some Morpho-physiological Attributes. , 2012; 14(7): 1579-1590.

Characterization of an Indigenous Isolate, Dunaliella tertiolecta ABRIINW-G3, from Gavkhooni Salt Marsh in Iran Based on Molecular and Some Morpho-physiological Attributes

Journal of Agricultural Science and Technology
Article 13, Volume 14, Issue 7, 2012, Pages 1579-1590    PDF (942.5 K)
Authors
N. Hosseinzadeh Gharajeh1; M. A. Hejazi* 1; S. Nazeri2; Abolfazl Barzegari1
1Branch of the Northwest and West region, Agricultural Biotechnology Research Institute of Iran (ABRII), Tabriz, Islamic Republic Iran.
2Department of Biotechnology, Faculty of Agriculture, Bu Ali Sina University, Hamedan, Islamic Republic Iran.
Abstract
Dunaliella is a green halotolerant microalga, which has several industrial applications e.g. β-carotene production. Identification of different Dunaliella species has been carried out by morpho-physiological and recently molecular studies. To achieve an improved understanding of taxonomy, these studies are required to be in linkage. The present study describes molecular and specific morpho-physiological properties of a Dunaliella isolate obtained from Gavkhooni salt marsh in Iran. Phylogenetic analysis of Internal Transcribed Spacer region demonstrated that the isolate was associated with different species except D. salina (CCAP 19/18 and 19/30) and D.viridis. 18S rDNA size of the isolate was identical to that of D. tertiolecta and intron-lacking strains of D. salina. 18S rDNA fingerprint profile and phylogenetic analysis revealed D. tertiolecta as the closest taxon to the isolate. Features of optimum growth salinity (1.5-3% w/v) and maximum carotenoid per cell (0.7 pg cell-1) were comparable with reported data for D. terrtiolecta. Morphological characteristics including the size and color of the cells, presence and location of stigma and refractile granules were similar to those of D. tertiolecta. Totally, considering molecular and morpho-physiological properties, the isolate was attributed to the species D. tertiolecta and was named as Dunaliella tertiolecta ABRIINW-G3.
Keywords
Carotenoid; Dunaliella; 18S rDNA; internal transcribed spacer; Optimum growth salinity
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