Dehghanikhah, Fahimeh, Shakarami, Jahanshir, Asoodeh, Ahmad (2021). Isolation and identification of lignin-degrading bacteria with laccase activity from the gut of the leopard moth, Zeuzera pyrina (Lepidoptera: Cossidae). , 10(1), 41-49. doi: 10.48311/jcp.2021.1518
Fahimeh Dehghanikhah; Jahanshir Shakarami; Ahmad Asoodeh. "Isolation and identification of lignin-degrading bacteria with laccase activity from the gut of the leopard moth, Zeuzera pyrina (Lepidoptera: Cossidae)". , 10, 1, 2021, 41-49. doi: 10.48311/jcp.2021.1518
Dehghanikhah, Fahimeh, Shakarami, Jahanshir, Asoodeh, Ahmad (2021). 'Isolation and identification of lignin-degrading bacteria with laccase activity from the gut of the leopard moth, Zeuzera pyrina (Lepidoptera: Cossidae)', , 10(1), pp. 41-49. doi: 10.48311/jcp.2021.1518
Dehghanikhah, Fahimeh, Shakarami, Jahanshir, Asoodeh, Ahmad Isolation and identification of lignin-degrading bacteria with laccase activity from the gut of the leopard moth, Zeuzera pyrina (Lepidoptera: Cossidae). , 2021; 10(1): 41-49. doi: 10.48311/jcp.2021.1518

Isolation and identification of lignin-degrading bacteria with laccase activity from the gut of the leopard moth, Zeuzera pyrina (Lepidoptera: Cossidae)

Journal of Crop Protection
Article 4, Volume 10, Issue 1, 2021, Pages 41-49    PDF (571.38 K)
Document Type: Original Research
DOI: 10.48311/jcp.2021.1518
Authors
Fahimeh Dehghanikhah1; Jahanshir Shakarami* 1; Ahmad Asoodeh2
1Department of Plant Protection, Faculty of Agriculture, Lorestan University, Khorramabad, Iran.
2Department of Chemistry, Faculty of Science, Ferdowsi University of Mashhad, Mashhad, Iran.
Abstract
Gut bacterial symbionts have an essential role in the nutrition and fitness of xylophagous insects. These bacteria produce several enzymes like cellulase and laccase which are important in industrial applications. In this study, laccase-producing bacteria were isolated and identified from the gut of the wood borer leopard moth. Four novel laccase positive strains were isolated using guaiacol-containing agar plates. Among the strains of dc4f, le2f, lc2, and lb8, the strain le2f displayed high laccase activity of 0.059 U ml-1 toward syringaldazine as a typical laccase substrate. The isolates were identified based on biochemical tests and 16S rRNA gene sequencing analyses. Nucleotide BLAST analyses of 16S rRNA gene sequence exhibited that the strains of dc4f, lb8, lc2, and le2f, had the most similarity (with more than 98% identity) with Enterobacter sp. strain W-6 16S (ACCN: MK505390), Serratia liquefaciens strain N112 (ACCN: MK629784), Brevibacterium sp. strain 773 (ACCN: MH777897) and Staphylococcus sciuri strain KSI 708 (ACCN: KC113150), respectively. Overall, the current study is the first research on alkaliphilic bacterial strains from the gut of leopard moth with laccase activity.
Keywords
Biological degradation; Lignocellulose; Insect symbionts; Delignification
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