Siosemarde, A., Osmani, Z., Bahramnezhad, B., Vahabi, Kh., Rouhi, Ebrahim (2012). Identification of AFLP Marker Associated with Stress Tolerance Index in Sardari Wheat Ecotypes. , 14(3), 629-643.
A. Siosemarde; Z. Osmani; B. Bahramnezhad; Kh. Vahabi; Ebrahim Rouhi. "Identification of AFLP Marker Associated with Stress Tolerance Index in Sardari Wheat Ecotypes". , 14, 3, 2012, 629-643.
Siosemarde, A., Osmani, Z., Bahramnezhad, B., Vahabi, Kh., Rouhi, Ebrahim (2012). 'Identification of AFLP Marker Associated with Stress Tolerance Index in Sardari Wheat Ecotypes', , 14(3), pp. 629-643.
Siosemarde, A., Osmani, Z., Bahramnezhad, B., Vahabi, Kh., Rouhi, Ebrahim Identification of AFLP Marker Associated with Stress Tolerance Index in Sardari Wheat Ecotypes. , 2012; 14(3): 629-643.

Identification of AFLP Marker Associated with Stress Tolerance Index in Sardari Wheat Ecotypes

Journal of Agricultural Science and Technology
Article 17, Volume 14, Issue 3, 2012, Pages 629-643    PDF (889.17 K)
Authors
A. Siosemarde* 1; Z. Osmani2; B. Bahramnezhad1; Kh. Vahabi1; Ebrahim Rouhi3
1Department of Agronomy and Plant Breeding, Faculty of Agriculture, University of Kurdistan, Sanandaj, Islamic Republic of Iran.
2Department of Agronomy and plant breeding, Faculty of Agriculture, University of Zabol, Islamic Republic of Iran.
3Agricultural Research Institute of Kurdistan, Sanandaj, Islamic Republic of Iran.
Abstract
Sardari is one of the most important landraces of common wheat (Triticum aestivum L.) that is mainly cultivated in drylands and mountainous area of Iran. Moreover, it shows a high level of genetic diversity. In the present research a total of 73 Sardari wheat ecotypes were evaluated for drought tolerance. Genetic diversity was analyzed using amplified fragment length polymorphism (AFLP) marker based on three pairs of primer combinations. Of the 2,431 AFLP bands detected, 1,582 (73.92%) were polymorphic. Cluster analysis divided all ecotypes into eight major groups. Ecotypes also showed genetic diversity for drought tolerance and were classified into three groups. The first group consisted of forty-two of the 73 landraces and had a low stress tolerance index (STI), ranging from 0.165 to 0.401, while the second (23 landraces) and the third group (7 landraces) had a medium and high STI ranging from 0.425 to 0.640 and 0.662 to 0.817, respectively. Discriminant analysis (DA) identified twenty-four markers selected from 218 AFLP markers that accounted for the difference between the three phenotypic groups. By using the selected markers, DA validated the phenotypic grouping, with a zero error rate. The results showed a high degree of genetic diversity between the Sardari ecotypes, suggesting that Sardari can be used as a germplasm source for wheat improvement toward releasing more desirable cultivars.
Keywords
AFLP; Discriminate analysis; Genetic diversity; Sardari wheat; STI
References
Statistics
Article View: 148
PDF Download: 70
Statistics
Number of Journals45
Number of Issues2,162
Number of Articles24,582
Article View20,478,850
PDF Download16,303,721