Bayati, Sh., Shams-bakhsh, M., Moini, A. (2011). Elimination of Grapevine Virus A (GVA) by Cryotherapy and Electrotherapy. , 13(3), 442-450.
Sh. Bayati; M. Shams-bakhsh; A. Moini. "Elimination of Grapevine Virus A (GVA) by Cryotherapy and Electrotherapy". , 13, 3, 2011, 442-450.
Bayati, Sh., Shams-bakhsh, M., Moini, A. (2011). 'Elimination of Grapevine Virus A (GVA) by Cryotherapy and Electrotherapy', , 13(3), pp. 442-450.
Bayati, Sh., Shams-bakhsh, M., Moini, A. Elimination of Grapevine Virus A (GVA) by Cryotherapy and Electrotherapy. , 2011; 13(3): 442-450.

Elimination of Grapevine Virus A (GVA) by Cryotherapy and Electrotherapy

Journal of Agricultural Science and Technology
Article 13, Volume 13, Issue 3, 2011, Pages 442-450    PDF (165.58 K)
Authors
Sh. Bayati1; M. Shams-bakhsh* 2; A. Moini1
1Department of Plant pathology, Faculty of Agriculture, Tarbiat Modares University, Tehran, Islamic Republic of Iran.
2Department of Plant Protection, Faculty of Agriculture, Tarbiat Modares University, Tehran, Islamic Republic of Iran.
Abstract
The incidence of grapevine virus A (GVA) is reported from almost all of the major
grapevine growing regions in Iran. Grapevine is vegetatively propagated by rooting of
cuttings or grafting. In such plants, viral diseases are transmitted from stock plants to the
progeny. Therefore, the control of grapevine viruses can be achieved primarily through
production of healthy stock plants. In the present research, cryotherapy and
electrotherapy were employed for elimination of GVA from naturally infected vine (Vitis
vinifera L. cv Black) and their efficiency was compared. In cryotherapy, 59% of the shoot
tips survived and regenerated into whole plants, of which 42% were free of GVA detected
by RT-PCR. In the electrotherapy, the effects of electric current value and treatment
duration were investigated on plant survival and virus elimination. The best results were
obtained by using 30 milliamps (mA) for 15 minutes. With this treatment, survival and
virus-free frequencies were about 62% and 40%, respectively. This is the first report of
electrotherapy of grapevine shoot tips as a potential tool for GVA elimination. The results
showed that cryotherapy was a more efficient and convenient protocol than
electrotherapy for elimination of GVA from infected grapevine.
Keywords
RT-PCR; Cryotherapy; Electrotherapy; Shoot tips; Vitis
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