Zhang, D. L., Gao, A. L., Li, Y. G., Su, Y. R., He, S. B., Li, S. P. (2014). Molecular Characterization and Phylogenetic Analysis of Novel α-gliadin Genes from Triticum dicoccoides L.. , 16(1), 203-215.
D. L. Zhang; A. L. Gao; Y. G. Li; Y. R. Su; S. B. He; S. P. Li. "Molecular Characterization and Phylogenetic Analysis of Novel α-gliadin Genes from Triticum dicoccoides L.". , 16, 1, 2014, 203-215.
Zhang, D. L., Gao, A. L., Li, Y. G., Su, Y. R., He, S. B., Li, S. P. (2014). 'Molecular Characterization and Phylogenetic Analysis of Novel α-gliadin Genes from Triticum dicoccoides L.', , 16(1), pp. 203-215.
Zhang, D. L., Gao, A. L., Li, Y. G., Su, Y. R., He, S. B., Li, S. P. Molecular Characterization and Phylogenetic Analysis of Novel α-gliadin Genes from Triticum dicoccoides L.. , 2014; 16(1): 203-215.

Molecular Characterization and Phylogenetic Analysis of Novel α-gliadin Genes from Triticum dicoccoides L.

Journal of Agricultural Science and Technology
Article 17, Volume 16, Issue 1, 2014, Pages 203-215    PDF (931.46 K)
Authors
D. L. Zhang; A. L. Gao; Y. G. Li; Y. R. Su; S. B. He; S. P. Li*
College of Life Science, Henan University, Kaifeng, 475001, Henan, People's Republic of China.
Abstract
The quality traits of Triticum dicoccoides KU-13441 (Triticum turgidum L. var. dicoccoides (AABB, 2n= 4x= 28)) were analyzed by mixograph, and the results showed that T. dicoccoides KU-13441 had desirable qualities in gluten strength and flour stirring tolerance. Subsequently, seventeen novel full-ORF α-gliadin genes and thirty-five pseudogenes were cloned and sequenced from T. dicoccoides KU-13441. Among the 17 novel full-ORF α-gliadin genes, the putative proteins of Gli2-TD-44 and Gli2-TD-46 contained an extra cysteine residue, located in the first nonrepetitive region and N-terminal repetitive domain, respectively, rather than in the second nonrepetitive region like other α-gliadins. Prokaryotic expression analysis and western-blotting indicated that these two α-gliadin genes could be successfully expressed under the control of T7 promoter. According to the varying numbers of 4 typical coeliac disease toxic peptides and glutamine residues in the two ployglutamine domains among the 17 α-gliadins, Gli2-TD-39, Gli2-TD-46 and Gli2-TD-47 genes were assigned to sub-genome B and other 14 genes were assigned to sub-genome A. Phylogenetic analysis including two S-genome species, Aegilops longissima (Sl) and Aegilops speltoides (S) revealed that the α-gliadin sequences of the B genome in T. dicoccoides had closer genetic relationship with those from Ae. speltoides. This implies that Ae. speltoides might participate in the origin of wheat B genome.
Keywords
α-gliadin genes; Molecular characterization; Phylogenetic analysis; Wild emmer wheat
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