Zolfaghari, Maryam, Ghadamyari, Mohammad, Hassan Sajedi, Reza (2019). Resistance mechanisms of a field population of diamond back moth, Plutella xylostella (Lepidoptera: Plutellidae) to current organophosphate pesticides. , 8(4), 403-416. doi: 10.48311/jcp.2019.1445
Maryam Zolfaghari; Mohammad Ghadamyari; Reza Hassan Sajedi. "Resistance mechanisms of a field population of diamond back moth, Plutella xylostella (Lepidoptera: Plutellidae) to current organophosphate pesticides". , 8, 4, 2019, 403-416. doi: 10.48311/jcp.2019.1445
Zolfaghari, Maryam, Ghadamyari, Mohammad, Hassan Sajedi, Reza (2019). 'Resistance mechanisms of a field population of diamond back moth, Plutella xylostella (Lepidoptera: Plutellidae) to current organophosphate pesticides', , 8(4), pp. 403-416. doi: 10.48311/jcp.2019.1445
Zolfaghari, Maryam, Ghadamyari, Mohammad, Hassan Sajedi, Reza Resistance mechanisms of a field population of diamond back moth, Plutella xylostella (Lepidoptera: Plutellidae) to current organophosphate pesticides. , 2019; 8(4): 403-416. doi: 10.48311/jcp.2019.1445

Resistance mechanisms of a field population of diamond back moth, Plutella xylostella (Lepidoptera: Plutellidae) to current organophosphate pesticides

Journal of Crop Protection
Article 3, Volume 8, Issue 4, 2019, Pages 403-416    PDF (2.25 M)
DOI: 10.48311/jcp.2019.1445
Authors
Maryam Zolfaghari* 1; Mohammad Ghadamyari1; Reza Hassan Sajedi2
1Department of Plant Protection, Faculty of Agricultural Sciences, University of Guilan, Rasht, Iran.
2Department of Biochemistry, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran.
Abstract
The toxicological and biochemical properties of four organophosphate (OP) insecticides, chlorpyrifos, diazinon, phosalone and dichlorvos, were examined in terms of the diamond back moth, Plutella xylostella (L.) susceptible (Gu-S) and resistant (Kar-R) to OPs. The Kar-R population had significantly high resistance to chlorpyriphos (69.3 fold), medium resistance to diazinon (14.49-fold) and phosalone (10.3-fold), and had less resistance to dichlorvos (5.17-fold) compared to Gu-S population. DEM and TPP reduced Chlopyrifos resistance of Kar-R population as an inhibitor of glutathione S-transferase (GST) and esterases (EST), respectively. Biochemical studies clarified that GST and EST kinetic parameters in the Kar-R population were significantly higher than parameters of Gu-S population. Moreover, this study examined the Kinetics of hydrolysis of acetylthiocholine iodide, butyrylthiocholine iodide as artificial substrates by AChE of resistant and susceptible population. IC50 of monocrotophos, neostigmine bromide and eserine were also determined on AChE of resistant and susceptible populations. Kinetic analysis and inhibition tests indicated that an alteration in AChE of Kar-R population has an effect on both kinetic and inhibition results. The results distinctly showed that multiple mechanisms such as GST, esterases and altered AChE created chlorpyrifos resistance in the Kar-R and insensitivity of AChE is a significant factor for resistance to conventional OP compounds.
Keywords
Plutella xylostella; organophosphate pesticides; biochemical mechanisms; Detoxification enzymes; Acetylcholinesterase
References
Statistics
Article View: 137
PDF Download: 77
Statistics
Number of Journals45
Number of Issues2,160
Number of Articles24,577
Article View19,975,752
PDF Download16,098,508