Davoodi, Abbas, Panjekeh, Naser, Moslemkhani, Kobra, Taheri, Abdolhossein (2019). Detection and molecular characterization of tomato big bud disease in Qazvin province. , 8(4), 379-388. doi: 10.48311/jcp.2019.1443
Abbas Davoodi; Naser Panjekeh; Kobra Moslemkhani; Abdolhossein Taheri. "Detection and molecular characterization of tomato big bud disease in Qazvin province". , 8, 4, 2019, 379-388. doi: 10.48311/jcp.2019.1443
Davoodi, Abbas, Panjekeh, Naser, Moslemkhani, Kobra, Taheri, Abdolhossein (2019). 'Detection and molecular characterization of tomato big bud disease in Qazvin province', , 8(4), pp. 379-388. doi: 10.48311/jcp.2019.1443
Davoodi, Abbas, Panjekeh, Naser, Moslemkhani, Kobra, Taheri, Abdolhossein Detection and molecular characterization of tomato big bud disease in Qazvin province. , 2019; 8(4): 379-388. doi: 10.48311/jcp.2019.1443

Detection and molecular characterization of tomato big bud disease in Qazvin province

Journal of Crop Protection
Article 1, Volume 8, Issue 4, 2019, Pages 379-388    PDF (743.17 K)
Document Type: Original Research
DOI: 10.48311/jcp.2019.1443
Authors
Abbas Davoodi1; Naser Panjekeh1; Kobra Moslemkhani* 2; Abdolhossein Taheri3
1Department of Plant Protection, Faculty of Agriculture, Zabol University, Zabol, Iran.
2Seed and Plant Certification and Registration Institute, AREEO, Karaj, Iran.
3Faculty of Plant Production, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran.
Abstract
Some samples were collected from tomato fields in Qazvin from tomato plants with big bud symptoms such as plant droop and purplish vein under the leaf, enlarged and sac-like pistils and malformed buds. DNA was extracted from the veins and vascular tissues of the plant with CTAB-based methods. In symptomatic plants, DNA fragments of 1800 and 1200bp were amplified by PCR using P1/P7, R16F2n/R16R2 primers. Restriction fragment length polymorphism (RFLP) analysis of nested R16F2n/R16R2 primed PCR product (1200bp) showed that the tomato big bud phytoplasma from Qazvin (TOM-Qazvin) is a member of clover proliferation (16SrVI). Phylogenetic analysis of 16SrRNA and putative restriction site analysis of the R16F2n/R16R2 primed sequence classified TOM phytoplasma in clover proliferation (16SrVI) group and belonged to subgroup 16SrVI-A. Virtual RFLP by using 1200bp sequencing of 16SRNA and 17 restriction enzymes confirmed that TOM-Qazvin belonged to the subgroup 16SrVI-A and16SrVI group. To our knowledge, this is the first report of tomato big bud disease in Qazvin province.
Keywords
big bud; iphyclassifier; RFLP; Tomato; Phytoplasma
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