Molecular characterization of a 16SrIX phytoplasma associated with Convolvulus glomeratus witches' broom and with an insect vector in Iran | ||
| Journal of Crop Protection | ||
| Article 3, Volume 7, Issue 4, 2018, Pages 387-393 PDF (751.52 K) | ||
| Document Type: Brief Communication | ||
| DOI: 10.48311/jcp.2018.1402 | ||
| Authors | ||
| Mehrnoosh Nikooei1; Chamran Hemmati* 2 | ||
| 1Department of Agriculture, Minab Higher Education Center, University of Hormozgan, Bandar Abbas, Iran. | ||
| 2Plant Protection Research Group, University of Hormozgan, Bandar Abbas, Iran. | ||
| Abstract | ||
| During a survey in 2016, Convolvulus glomeratus showing symptoms resembling those caused by phytoplasmas were observed in Bandar Abbas, Hormozgan province, Iran. These plants were examined for phytoplasma presence by nested-PCR assays using universal primer pair P1/P7 followed by R16F2n/R16R2. All the infected plants indicated positive results using universal primer pairs of P1/P7 followed by R16F2n/R16R2. Results of phylogenetic and virtual RFLP analyses of the 16S rRNA gene sequences revealed that the phytoplasma associated with Convolvulus glomeratus witches' broom (CgWB) was a strain of 'Candidatus Phytoplasma phoenicium'. The phytoplasma was successfully transmitted to healthy plants by leafhopper, Orosius albicinctus (Distant) which account as a vector of this phytoplasma. This is the first report on the presence of ‘Candidatus Phytoplasma phoenicium’ (phytoplasma group 16SrIX-J) in C. glomeratus and its insect vector in Iran. | ||
| Keywords | ||
| 16SrIX; Convolvulus glomeratus; Orosius albicinctus; Bandar Abbas; witches' broom | ||
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