Kordi, Masoumeh, Farrokhi, Naser, Masoudi, Abolfazl, Derakhshan Shadmehri, Ali, Gharanjik, Shahrokh (2015). Expression analyses of some Beauveria bassiana genes in response to cuticles of four different insects. , 4(5), 675-690. doi: 10.48311/jcp.2015.1249
Masoumeh Kordi; Naser Farrokhi; Abolfazl Masoudi; Ali Derakhshan Shadmehri; Shahrokh Gharanjik. "Expression analyses of some Beauveria bassiana genes in response to cuticles of four different insects". , 4, 5, 2015, 675-690. doi: 10.48311/jcp.2015.1249
Kordi, Masoumeh, Farrokhi, Naser, Masoudi, Abolfazl, Derakhshan Shadmehri, Ali, Gharanjik, Shahrokh (2015). 'Expression analyses of some Beauveria bassiana genes in response to cuticles of four different insects', , 4(5), pp. 675-690. doi: 10.48311/jcp.2015.1249
Kordi, Masoumeh, Farrokhi, Naser, Masoudi, Abolfazl, Derakhshan Shadmehri, Ali, Gharanjik, Shahrokh Expression analyses of some Beauveria bassiana genes in response to cuticles of four different insects. , 2015; 4(5): 675-690. doi: 10.48311/jcp.2015.1249

Expression analyses of some Beauveria bassiana genes in response to cuticles of four different insects

Journal of Crop Protection
Article 8, Volume 4, Issue 5, 2015, Pages 675-690    PDF (389.48 K)
DOI: 10.48311/jcp.2015.1249
Authors
Masoumeh Kordi1; Naser Farrokhi* 2; Abolfazl Masoudi3; Ali Derakhshan Shadmehri3; Shahrokh Gharanjik3
1Biotechnology Research Department, Shahrood University, Shahrood, Iran.
2Department of Biotechnology Engineering, Faculty of Energy Engineering and New Technologies, Shahid Beheshti University, Tehran, Iran.
3Department of Agronomy and Plant Breeding, Faculty of Agriculture, Shahrood University, Sahrood, Iran.
Abstract
Beauveria spp. as entomopathogenic fungi have extremely diverse insect host ranges.Here, a comparative transcript analysis of a Bassiasin I (BSNI), a Dipeptidyl peptidase (DPP), a Cytochrom P450 monooxygenase (CYP52), a Mitogen activated protein kinase (MAPK), and a Neuronal calcium sensor 1 (NCS) in two isolates of Beauveria bassiana., with low and high virulence, were carried out. For this, induction media, containing isolated cuticles from four insects, and a check medium lacking any cuticular extract were prepared and isolates were allowed to grow on them. Interestingly, up-regulation of BSNI and DPP in induction media was noted in comparison to the check, following normalization with γ-ACTIN. CYP52 was up-regulated in synthetic medium in comparison to cuticular extract. MAPK expression was greater in most virulent isolate once compared with the low virulent isolate. The expression of MAPK was greater in the presence of Caliptamus italicus cuticles and synthetic medium as opposed to others. NCS expression remained on changed in all media tested and can be suggested as a reference gene in transcriptomics of Beauveria spp. genes in future.
Keywords
Bassiasin I; Dipeptidyl peptidase; Cytochrome P450 monooxygenase; Mitogen activated protein kinase; Neuronal calcium sensor
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