Kheiri, Hamid-Reza, Motallebi, Mostafa, Zamani, Mohammad Reza, Deljo, Ali (2014). Beta glucanase (Bgn13.1) expressed in transgenic Brassica napus confers antifungal activity against Sclerotinia sclerotiorum. , 3(1), 31-42. doi: 10.48311/jcp.2014.1115
Hamid-Reza Kheiri; Mostafa Motallebi; Mohammad Reza Zamani; Ali Deljo. "Beta glucanase (Bgn13.1) expressed in transgenic Brassica napus confers antifungal activity against Sclerotinia sclerotiorum". , 3, 1, 2014, 31-42. doi: 10.48311/jcp.2014.1115
Kheiri, Hamid-Reza, Motallebi, Mostafa, Zamani, Mohammad Reza, Deljo, Ali (2014). 'Beta glucanase (Bgn13.1) expressed in transgenic Brassica napus confers antifungal activity against Sclerotinia sclerotiorum', , 3(1), pp. 31-42. doi: 10.48311/jcp.2014.1115
Kheiri, Hamid-Reza, Motallebi, Mostafa, Zamani, Mohammad Reza, Deljo, Ali Beta glucanase (Bgn13.1) expressed in transgenic Brassica napus confers antifungal activity against Sclerotinia sclerotiorum. , 2014; 3(1): 31-42. doi: 10.48311/jcp.2014.1115

Beta glucanase (Bgn13.1) expressed in transgenic Brassica napus confers antifungal activity against Sclerotinia sclerotiorum

Journal of Crop Protection
Article 4, Volume 3, Issue 1, 2014, Pages 31-42    PDF (303.57 K)
DOI: 10.48311/jcp.2014.1115
Authors
Hamid-Reza Kheiri1; Mostafa Motallebi* 1; Mohammad Reza Zamani1; Ali Deljo2
1National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran, Iran.
2Department. of Agricultural Biotechnology, Faculty of Agriculture, Bu-Ali Sina University, Hamadan, Iran.
Abstract
Brassica napus is an important oilseed crop and the yield loss due to fungal disease stem rot caused by Sclerotinia sclerotiorum is a serious problem in cultivation of this crop. The pathogenesis-related (PR) protein, glucanase, hydrolyzes a major cell wall component, glucan, of the pathogenic fungi and acts as a plant defense barrier. In this study, a β-1,3-glucanase (bgn13.1) gene was isolated from the biocontrol fungus Trichoderma virens-10 (showing the high β-glucanase activity) and cloned in pUC19 cloning vector. The cloned fragment was confirmed by molecular analysis and showed to contain two short introns, 52 and 57 bp and an open reading frame coding 761 amino acids. The bgn13.1 gene was over-expressed under the CaMV35S promoter in B. napus, R line Hyola 308. Transformation of cotyledonary petioles was achieved by pBIKH1 containing bgn13.1 gene via Agrobacterium tumefaciens LBA4404. The insertion of transgene was verified by the polymerase chain reaction (PCR) and genomic DNA Southern dot blotting in T0 generation. RT-PCR analysis indicated that the transgenic canola plants were able to transcribe the β-1,3 glucanase gene. Also, we used transgenic over-expression approach in order to investigate antifungal activity of expressed Bgn13.1 on S. sclerotiorum. The heterologous expressed Bgn13.1 of line # 7 and line # 10 compared with other lines showed stronger inhibition against hyphal growth of S. sclerotiorum with
Keywords
bgn13.1; Brassica napus; glucanase enzyme; Sclerotinia sclerotiorum; Transgenic plants; Trichoderma virens
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