Mousavi Fard, Seyed Hossein, Merat, Shahin, Shahzamani, Kiana, Ghanbari, Reza, Yahoo, Neda, Sabahi, Farzaneh (2014). Development of a SYBR green real time multiplex RT-PCR technique for simultaneous detection of HCV and GBV-C Co-infection in plasma samples. , 1(1), 41-49.
Seyed Hossein Mousavi Fard; Shahin Merat; Kiana Shahzamani; Reza Ghanbari; Neda Yahoo; Farzaneh Sabahi. "Development of a SYBR green real time multiplex RT-PCR technique for simultaneous detection of HCV and GBV-C Co-infection in plasma samples". , 1, 1, 2014, 41-49.
Mousavi Fard, Seyed Hossein, Merat, Shahin, Shahzamani, Kiana, Ghanbari, Reza, Yahoo, Neda, Sabahi, Farzaneh (2014). 'Development of a SYBR green real time multiplex RT-PCR technique for simultaneous detection of HCV and GBV-C Co-infection in plasma samples', , 1(1), pp. 41-49.
Mousavi Fard, Seyed Hossein, Merat, Shahin, Shahzamani, Kiana, Ghanbari, Reza, Yahoo, Neda, Sabahi, Farzaneh Development of a SYBR green real time multiplex RT-PCR technique for simultaneous detection of HCV and GBV-C Co-infection in plasma samples. , 2014; 1(1): 41-49.

Development of a SYBR green real time multiplex RT-PCR technique for simultaneous detection of HCV and GBV-C Co-infection in plasma samples

Molecular and Biochemical Diagnosis Journal
Article 5, Volume 1, Issue 1, 2014, Pages 41-49    PDF (169.91 K)
Authors
Seyed Hossein Mousavi Fard1; Shahin Merat2; Kiana Shahzamani3; Reza Ghanbari4; Neda Yahoo3; Farzaneh Sabahi* 1
1Department of Virology, Faculty of Medical Sciences, TarbiatModares University, Tehran, Iran
2Digestive Disease Research Center, Tehran University of Medical Sciences, Tehran, Iran
3Department of Biology, Faculty of Sciences, Lorestan University, Khorramabad, Iran Department of Virology, Faculty of Medical Sciences, TarbiatModares University, Tehran, Iran
4Department of Virology, Faculty of Medical Sciences, TarbiatModares University, Tehran, Iran Department of Biology, Faculty of Sciences, Lorestan University, Khorramabad, Iran
Abstract
Background:Accumulative research is in progress to clarify clinical aspects of GBV-C. The possibility of interaction between HCV and GBV-C as well as its consequence on development of liver diseases is the most important clinical aspect which encourages researchers to develop a rapid and cost effective technique for simultaneous detection of both viruses. Methods: In this study, a SYBR Green real time multiplex RT-PCR technique as a new economical and sensitive method was designed and validated for simultaneous detection of HCV/GBV-C in HCV positive plasma samples. SYBR green real time RT-PCR technique optimization was performed separately for each virus. Multiplex PCR was established next. Standard sera with known concentrations of HCV RNA and dual HCV/GBV-C positive control samples along with negative control samples were used to validate the assay. Results and Conclusions: Fifty six non cirrhotic HCV positive plasma samples [29 of genotype 3a and 27 of genotype 1a] were collected from patients before receiving treatment. 20.6% of genotype 3a and 18.7% of genotype 1a showed HCV/GBV-C co-infection. As a result, 19.6% of 56 samples had HCV/GBV-C co-infection that was compatible with other results from all over the world. SYBR Green real time multiplex RT-PCR technique can be used to detect HCV/GBV-C co-infection in plasma samples. Furthermore, with application of this method more time and cost could be saved in clinical-research settings.
Keywords
HCV; GBV-C; SYBR Green real time; Rapid detection; multiplex RT- PCR
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