Evaluation of the Relative Frequency of Carbapenemase Genes by Phenotypic and Genotypic Methods in Pseudomonas aeruginosa Isolates from Patients with Open Heart Surgery in Iran | ||
| Infection Epidemiology and Microbiology | ||
| Article 6, Volume 9, Issue 1, 2023, Pages 55-62 PDF (464.39 K) | ||
| Document Type: Original Research | ||
| DOI: 10.52547/iem.9.1.55 | ||
| Authors | ||
| Maryam Mokhtari1; Ali Mojtahedi* 1; Nejat Mahdieh2; Alireza Jafari3; Zahra Atrkar Roushan4; Mohammad Javad Arya5 | ||
| 1Department of Microbiology, School of Medicine, Guilan University of Medical Sciences, Rasht, Iran | ||
| 2Cardiogenetic Research Center, Rajaei Cardiovascular Medical and Research Center, Iran University of Medical Sciences, Tehran, Iran | ||
| 3Urology Research Center, Razi Hospital, School of Medicine, Guilan University of Medical Sciences, Rasht, Iran | ||
| 4School of Medicine, Guilan University of Medical Sciences, Rasht, Iran | ||
| 5Anatomical and clinical Pathologist, Fellowship of dermatopathology, Head of Sina Pathobiology Lab, Yazd, Iran. | ||
| Abstract | ||
| Backgrounds: Carbapenem resistance among Pseudomonas aeruginosa strains is alarming. This study aimed to investigate the relative frequency of carbapenem-resistant P. aeruginosa strains by phenotypic and genotypic methods. Materials & Methods: The antibiotic susceptibility pattern of 60 P. aeruginosa isolates was determined by disk diffusion method (Kirby-Bauer). BD Phoenix automated microbiology system was used to identify carbapenem-resistant isolates, and the minimum inhibitory concentration (MIC) was determined using E-Test. In addition, mCIM (modified carbapenem inactivation method) phenotypic test was performed to evaluate carbapenem resistance genes in P. aeruginosa isolates. The prevalence of metallo-beta-lactamase (MβL) genes in carbapenem-resistant P. aeruginosa isolates was determined using conventional polymerase chain reaction (PCR). Findings: The frequency of carbapenem-resistant P. aeruginosa isolates was 36% (22 of 60). The highest resistance was observed to imipenem and meropenem (36.6%), and the highest sensitivity was observed to amikacin (75%). All carbapenem-resistant P. aeruginosa isolates were confirmed by the BD Phoenix automated system (MIC> 8 µg/mL for imipenem and meropenem), E-test (MIC ˂32 µg/mL), and mCIM assay (the growth inhibition zone diameter was 6-8 mm). In carbapenem-resistant P. aeruginosa isolates, the frequency of blaVIM, blaIMP, and blaSPM genes was 9.1% (2 of 22), 4.5% (1 of 22), and 4.5% (1 of 22), respectively. BlaKPC and blaNDM genes were not found in any of the isolates. Conclusion: Based on the present study results, all phenotypic tests used to identify carbapenemase-producing isolates had the same sensitivity (100%) and specificity (100%). | ||
| Keywords | ||
| Carbapenemases; Pseudomonas aeruginosa; Phenotypic; Bacterial sensitivity tests | ||
| References | ||
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