Improvement of Salinity Tolerance Indices and Regulation of Na+ and K+ Homeostasis in Hashemi Rice Mutants | ||
| Journal of Agricultural Science and Technology | ||
| Article 11, Volume 27, Issue 5, July and August 2025, Pages 1123-1136 PDF (6.69 M) | ||
| DOI: 10.48311/jast.2025.24126 | ||
| Authors | ||
| Leila Khazaie; Reza Shirzadian-Khorramabad* | ||
| Department of Agricultural Biotechnology, Faculty of Agricultural Sciences, University of Guilan, Rasht, Islamic Republic of Iran. | ||
| Abstract | ||
| In this study, we evaluated the effectiveness of gene expression changes on ion homeostasis comprising Salt Overly Sensitive (SOS1) and vacuolar Na+/H+ antiporter (NHX1) along with ion and proline content measurement in Hashemi rice mutants at Rice Research Institute of Iran, in 2018-2019. Tolerant mutant genotypes (em4hs290 and em4hs84) along with Hashemi parent cultivar, IR28 (sensitive), and FL478 (tolerant) seedlings were treated with 100 mM NaCl. Based on the results of growth indices, the seedling length of Hashemi cultivar and IR28 decreased considerably, about 44.7, and 44.2% reduction compared to the control, and the leaves progressively yellowed. Results showed that proline content and K+ and K+/Na+ ratio increased about ~2–3-fold in the tolerant genotypes than in the susceptible ones. Also, the overall amount of the OsNHX1 and SOS1 expression increased in tolerant genotypes compared to the susceptible ones. Accordingly, the compatible solute accumulation significantly advanced, resulting in improvement of ionic homeostasis and, probably, suppressed the stress. Moreover, the variable pattern of gene expression in the two salt-tolerant mutants (em4hs290 and em4hs84) and Hashemi parent showed that the induced mutation could increase the salt-tolerant in mutant genotypes through ionic and osmotic homeostasis. Generally, these tolerant mutant genotypes could be applied to develop salt-tolerant varieties in rice breeding programs, which can lead to production sustainability. | ||
| Keywords | ||
| Gene expression; Mutation; Stress Index | ||
| References | ||
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