Nasrollahi, Zahra, Roudbar Mohammadi, Shahla, Atyabi, Fatemeh, Zuhair Sarraf, Hassan, Yadegari, Mohammad Hossein, Esfandyari, Mehdi, Mollarazi, Esmail (2013). A Rapid Method for Extraction of Water Soluble β(1,3) Glucan from the Cell Wall of Candida albicans. , 16(1), 89-97.
Zahra Nasrollahi; Shahla Roudbar Mohammadi; Fatemeh Atyabi; Hassan Zuhair Sarraf; Mohammad Hossein Yadegari; Mehdi Esfandyari; Esmail Mollarazi. "A Rapid Method for Extraction of Water Soluble β(1,3) Glucan from the Cell Wall of Candida albicans". , 16, 1, 2013, 89-97.
Nasrollahi, Zahra, Roudbar Mohammadi, Shahla, Atyabi, Fatemeh, Zuhair Sarraf, Hassan, Yadegari, Mohammad Hossein, Esfandyari, Mehdi, Mollarazi, Esmail (2013). 'A Rapid Method for Extraction of Water Soluble β(1,3) Glucan from the Cell Wall of Candida albicans', , 16(1), pp. 89-97.
Nasrollahi, Zahra, Roudbar Mohammadi, Shahla, Atyabi, Fatemeh, Zuhair Sarraf, Hassan, Yadegari, Mohammad Hossein, Esfandyari, Mehdi, Mollarazi, Esmail A Rapid Method for Extraction of Water Soluble β(1,3) Glucan from the Cell Wall of Candida albicans. , 2013; 16(1): 89-97.

A Rapid Method for Extraction of Water Soluble β(1,3) Glucan from the Cell Wall of Candida albicans

Pathobiology Reserach
Article 8, Volume 16, Issue 1, 2013, Pages 89-97    PDF (502.15 K)
Authors
Zahra Nasrollahi1; Shahla Roudbar Mohammadi* 1; Fatemeh Atyabi2; Hassan Zuhair Sarraf3; Mohammad Hossein Yadegari1; Mehdi Esfandyari2; Esmail Mollarazi2
1Department of Medical Mycology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
2Nanotechnology Research Center, Faculty of Pharmacy, Tehran University, Tehran, Iran
3Department of Immunology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
Abstract
Objective: In recent decades, β-glucans have been used as important complementary and alternative medicines for numerous immunocompromised individuals and those with end stage of cancer terminal. The most active form of β-glucans is β(1,3)D-glucan and its most common source is cell wall of Candida albicans. Recently it has been introduced as a nano particle design to be used as a carrier for drug delivery. The current study researches a rapid method for the extraction of β(1,3)D-glucans. Methods: The present study was conducted atTarbiatModaresMedicalUniversity in 2012. Candida solubilized β-glucans were obtained by oxidation of the cell wall with sodium hypochlorite and sodium hydroxide. The particle part could be solubilized by treatment with dimethylsulfoxide (DMSO) and zymolyase digestion to extract β(1,3)D-glucan. The soluble fractions were lyophilized. We performed the Callose test to verify the presence of β(1,3)D-glucans. Solubilized fractions were dissolved in D2O and 1H-NMR spectra were measured. Results: The soluble β(1,3)D-glucan fraction which was derived from 1 g of dried Candida albicans germ tube weighed 190 mg. β(1,3)D-glucan was verified by the Callose test and 1H-NMR test compared with Curdlan (standard). 1H-NMR spectra verified the existence of β(1,3)D-glucan in the final product. Conclusion: In the present study, extraction of β(1,3)D-glucan by oxidation of the cell wall using sodium hypochlorite yielded more pure β(1,3)D-glucans in comparison with other extraction methods. Thus it might represent a rapid method of extraction.
Keywords
β(1; 3)D-glucans; Oxidation of cell wall of Candida albicans; Enzymatic extraction
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