Saadati, Mojtaba, Nazarian, Shahram, Barati, Babak, Mehdizadeh, Hossein, Sadeghizadeh, Majid, Shirzad, Hadi Shirzad, Sadeghizadeh, Majid, Mosapour, Abbas (2008). Detection and identification of Leishmania parasites within sand flies using kDNA, rDNA and CPB loci. , 11(0), -.
Mojtaba Saadati; Shahram Nazarian; Babak Barati; Hossein Mehdizadeh; Majid Sadeghizadeh; Hadi Shirzad Shirzad; Majid Sadeghizadeh; Abbas Mosapour. "Detection and identification of Leishmania parasites within sand flies using kDNA, rDNA and CPB loci". , 11, 0, 2008, -.
Saadati, Mojtaba, Nazarian, Shahram, Barati, Babak, Mehdizadeh, Hossein, Sadeghizadeh, Majid, Shirzad, Hadi Shirzad, Sadeghizadeh, Majid, Mosapour, Abbas (2008). 'Detection and identification of Leishmania parasites within sand flies using kDNA, rDNA and CPB loci', , 11(0), pp. -.
Saadati, Mojtaba, Nazarian, Shahram, Barati, Babak, Mehdizadeh, Hossein, Sadeghizadeh, Majid, Shirzad, Hadi Shirzad, Sadeghizadeh, Majid, Mosapour, Abbas Detection and identification of Leishmania parasites within sand flies using kDNA, rDNA and CPB loci. , 2008; 11(0): -.

Detection and identification of Leishmania parasites within sand flies using kDNA, rDNA and CPB loci

Pathobiology Reserach
Article 4, Volume 11, Issue 0, 2008    PDF (316.54 K)
Authors
Mojtaba Saadati* 1; Shahram Nazarian2; Babak Barati2; Hossein Mehdizadeh2; Majid Sadeghizadeh3; Hadi Shirzad Shirzad4; Majid Sadeghizadeh3; Abbas Mosapour5
1Associated Professor, Department of Biology, Imam Houssein University, Tehran, Iran
2M.Sc., Department of Biology, Imam Houssein University, Tehran, Iran
3Associated Professor, Department of Genetics, Faculty of Basic Sciences, Tarbiat Modares University, Tehran, Iran
4Ph.D. Student, Department of Biology, Imam Houssein University, Tehran, Iran
5Ph.D. Student, Department of Clinical Biochemistry, School of Medical Sciences, Tarbiat Modares University, Tehran, Iran
Abstract
Objective: Molecular epidemiology of Visceral Leishmaniasis (VL) is currently used widely for different objectives such as vector incrimination studies.
Materials and Methods: In this study three different loci including kinetoplast DNA (kDNA), ribosomal DNA (rDNA), and Cystein protease B (CPB) of Leishmania parasite genome were used for detection and identification of natural infection of sand flies of Germi district of Ardebil province, the most important VL or Kala-azar foci in Iran.
Results: The results showed that the three loci of kDNA, rDNA and CPBs are respectively more appropriate for leptomonad infection/initial screening, identification of the L.donovani complex, and discrimination of the species complex. It was also verified that both members of the complex,
L. donovani and L. infantum, are present in the study area and are transmitted to the hosts by
Keywords
Multiplex PCR; kDNA; rDNA
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